Human Cathepsin G ELISA Kit | CTSG elisa kit

Human Cathepsin G ELISA Kit

Cat. Num. AAA17533

• Gene Names: CTSG; CG; CATG
• Reactivity: Human
• Synonyms: Cathepsin G; Human Cathepsin G ELISA Kit; CTSG (Cathepsin G)/CG/CATG; CTSG elisa kit

• Reactivity: Human
• Specificity: Specifically recognize CTSG, no obvious cross reaction with other analogues
• Sequence Length: 255
• Assay Type: Sandwich
• Samples: Serum, plasma, cell culture supernatant and other biological samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
• Inter-assay Precision: Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for CTSG elisa kit

Background: Cathepsin G is a serine protease which was first identified in the cytoplasmic azurophilic granules of neutrophil leukocytes. As a degradative enzyme, Cathepsin G acts both intracellularly to degrade ingested host pathogens and extracellularly to breakdown ECM components at inflammatory sites. Independent of its serine protease activity, Cathepsin G is also known to exert broad-spectrum antibacterial action against Gram-negative and - positive bacteria. Cathepsin G has been reported to play an important role in the pathophysiology of several diseases, such as chronic obstructive pulmonary disease (COPD), rheumatoid arthritis, cystic fibrosis, and other conditions clinically manifested by excessive inflammatory reactions.

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti CTSG antibody was precoated onto the 96-well plate. The biotin conjugated anti CTSG antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with CTSG conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CTSG in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

NCBI and Uniprot Product Information

• NCBI GI #: 4503149
• NCBI GeneID: 1511
• NCBI Accession #: NP_001902.1
• NCBI GenBank Nucleotide #: NM_001911.2
• Molecular Weight: 28,837 Da
• NCBI Official Full Name: cathepsin G preproprotein
• NCBI Official Synonym Full Names: cathepsin G
• NCBI Official Symbol: CTSG
• NCBI Official Synonym Symbols: CG; CATG
• NCBI Protein Information: cathepsin G
• UniProt Protein Name: Cathepsin G
• UniProt Gene Name: CTSG
• UniProt Synonym Gene Names: CG
• UniProt Entry Name: CATG_HUMAN