CMRL1066 10X w/o L-Glutamine Culture Media
CMRL1066 10X w/o L-Glutamine (Powder)
Purity
Cell Culture Grade
Synonyms
CMRL1066 10X w/o L-Glutamine; N/A; CMRL1066 10X w/o L-Glutamine (Powder); CMRL1066 10X w/o L-Glutamine culture media
Purity/Purification
Cell Culture Grade
Appearance
Light pink, homogenous , free flowing powder
Solubility
Orange turbid, incomplete at 10X Yellow , clear, complete at 1X
pH
3.7(10x)
3.9 (1X)
3.9 (1X)
Endotoxin
< 1 EU/mL
Directions per Liter of 10X
Dissolve 97.34g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 22g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Some precipitate may be observed due to excess concentration of salts. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving. Note: USB recommends the preparation of this product as a 1X solution.
Components shown as g/Liter (10X)
Inorganic Salts:
Calcium Chloride 2.00
Magnesium Sulfate, anhy 0.9769
Potassium Chloride 4.00
Sodium Acetate, anhy 0.50
Sodium Chloride 68.0
Sodium Phosphate Mono, anhy 1.22
Amino Acids:
L-Alanine 0.25
L-Arginine 0.5787
L-Aspartic Acid 0.30
L-Cysteine•HCL•H2O 2.60
L-Cystine 0.20
L-Glutamic Acid, Sodium 0.862
L-Glutamine Absent
Glycine 0.50
L-Histidine•HCl•H2O 0.20
trans-4-Hydroxy-L-Proline 0.10
L-Isoleucine 0.20
L-Leucine 0.60
L-Lysine•HCl 0.70
L-Methionine 0.15
Components shown as g/liter10X:
L-Phenylalanine 0.25
L-Proline 0.40
L-Serine 0.25
L-Threonine 0.30
L-Tryptophan 0.10
L-Tyrosine 0.40
L-Valine 0.25
Vitamins:
PABA 0.0005
L-Ascorbic Acid•Na 0.50
D-Biotin 0.0001
Choline Chloride 0.005
Coenzyme A•Na 0.025
Cocarboxylase 0.01
2'-Deoxyadenosine 0.10
2'-Deoxycytidine•HCl 0.116
2'-Deoxyguanosine 0.10
Folic Acid 0.0001
myo-Inositol 0.0005
5-Methyldeoxycytidine 0.001
ß-NAD 0.070
ß-NADP•Na 0.010
Nicotinic Acid 0.00025
Niacinamide 0.00025
D-Pantothenic Acid•Ca 0.0001
Pyridoxal•HCl 0.00025
Pyridoxine•HCl 0.00025
Riboflavin 0.0001
Flavin Adenine Dinucleotide•2Na 0.00106
Thiamine•HCl 0.0001
Other:
Cholesterol 0.002
Thymidine 0.10
D-Glucose 10.0
D-Glucuronic Acid•Na 0.0388
Glutathione 0.10
Phenol Red, Sodium 0.2124
Tween80 0.05
Uridine-5-Triphosphate•4Na 0.0113
Total: 97.34 g/Liter
Calcium Chloride 2.00
Magnesium Sulfate, anhy 0.9769
Potassium Chloride 4.00
Sodium Acetate, anhy 0.50
Sodium Chloride 68.0
Sodium Phosphate Mono, anhy 1.22
Amino Acids:
L-Alanine 0.25
L-Arginine 0.5787
L-Aspartic Acid 0.30
L-Cysteine•HCL•H2O 2.60
L-Cystine 0.20
L-Glutamic Acid, Sodium 0.862
L-Glutamine Absent
Glycine 0.50
L-Histidine•HCl•H2O 0.20
trans-4-Hydroxy-L-Proline 0.10
L-Isoleucine 0.20
L-Leucine 0.60
L-Lysine•HCl 0.70
L-Methionine 0.15
Components shown as g/liter10X:
L-Phenylalanine 0.25
L-Proline 0.40
L-Serine 0.25
L-Threonine 0.30
L-Tryptophan 0.10
L-Tyrosine 0.40
L-Valine 0.25
Vitamins:
PABA 0.0005
L-Ascorbic Acid•Na 0.50
D-Biotin 0.0001
Choline Chloride 0.005
Coenzyme A•Na 0.025
Cocarboxylase 0.01
2'-Deoxyadenosine 0.10
2'-Deoxycytidine•HCl 0.116
2'-Deoxyguanosine 0.10
Folic Acid 0.0001
myo-Inositol 0.0005
5-Methyldeoxycytidine 0.001
ß-NAD 0.070
ß-NADP•Na 0.010
Nicotinic Acid 0.00025
Niacinamide 0.00025
D-Pantothenic Acid•Ca 0.0001
Pyridoxal•HCl 0.00025
Pyridoxine•HCl 0.00025
Riboflavin 0.0001
Flavin Adenine Dinucleotide•2Na 0.00106
Thiamine•HCl 0.0001
Other:
Cholesterol 0.002
Thymidine 0.10
D-Glucose 10.0
D-Glucuronic Acid•Na 0.0388
Glutathione 0.10
Phenol Red, Sodium 0.2124
Tween80 0.05
Uridine-5-Triphosphate•4Na 0.0113
Total: 97.34 g/Liter
Preparation and Storage
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for CMRL1066 10X w/o L-Glutamine culture media
CMRL-1066 is a chemically defined medium developed in the late 1960's at the Connaugh Medical Research Laboratories. CMRL-1066 was designed initially for use with mouse L-cells in non-supplemented culture. Although developed for use in serum-free culture, CMRL-1066 can be supplemented with serum and used to support the growth of many cell types. Suitable for growing a wide variety of cell types, especially fibroblast-like cells.
Product Categories/Family for CMRL1066 10X w/o L-Glutamine culture media
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Product Notes
The CMRL1066 10X w/o L-Glutamine (Catalog #AAA14827) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CMRL1066 10X w/o L-Glutamine, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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