McCoy's 5A Medium w/L-Glutamine w/o Phenol Red Culture Media
McCoy's 5A Medium w/L-Glutamine w/o Phenol Red (Powder)
Purity
Cell Culture Grade
Synonyms
McCoy's 5A Medium w/L-Glutamine w/o Phenol Red; N/A; McCoy's 5A Medium w/L-Glutamine w/o Phenol Red (Powder); McCoy's 5A Medium w/L-Glutamine w/o Phenol Red culture media
Purity/Purification
Cell Culture Grade
Form/Format
Powder
Appearance
Pale Yellow, homogeneous, free flowing powder.
Solubility
Pale orange, clear, complete
pH
5.2
Endotoxin Level
< 1 EU/ml
Directions per Liter
Dissolve 11.9g in 800-900ml of ddH20 stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1 L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Vitamins
P-Aminobenzoic Acid: 0.001
Ascorbic Acid*Na: 0.0005625
D-Biotin: 0.0002
Choline Chloride: 0.005
Vitamin B-12: 0.002
Folic Acid: 0.01
myo-Inositol: 0.036
Nicotinic Acid: 0.0005
Niacinamide: 0.0005
D-Pantothenic Acid * Ca: 0.0002
Pyridoxal*HCI: 0.0005
Pyridoxine*HCI: 0.0005
Riboflavin: 0.0002
Thiamine * HCI: 0.0002
Ascorbic Acid*Na: 0.0005625
D-Biotin: 0.0002
Choline Chloride: 0.005
Vitamin B-12: 0.002
Folic Acid: 0.01
myo-Inositol: 0.036
Nicotinic Acid: 0.0005
Niacinamide: 0.0005
D-Pantothenic Acid * Ca: 0.0002
Pyridoxal*HCI: 0.0005
Pyridoxine*HCI: 0.0005
Riboflavin: 0.0002
Thiamine * HCI: 0.0002
Other
D-Glucose: 3.00
Glutathione: 0.0005
Peptone: 0.60
Glutathione: 0.0005
Peptone: 0.60
Components shown as Inorganic Salts
Calcium Chloride * 2H20: 0.1324 g/Liter
Magnesium Sulfate: 0.0976 g/Liter
Potassium Chloride: 0.40 g/Liter
Sodium Chloride: 6.46 g/Liter
Sodium Phosphate Monobasic: 0.504 g/Liter
Magnesium Sulfate: 0.0976 g/Liter
Potassium Chloride: 0.40 g/Liter
Sodium Chloride: 6.46 g/Liter
Sodium Phosphate Monobasic: 0.504 g/Liter
Amino Acids
L-Alanine: 0.01336 g/Liter
L-Arginine * HCI: 0.04214 g/Liter
L-Asparagine * H20: 0.04503 g/Liter
L-Aspartic Acid: 0.01997 g/Liter
L Cysteine, Free base: 0.02424 g/Liter
L-Glutamic Acid, Sodium: 0.02207 g/Liter
L-Glutamine: 0.21915 g/Liter
Glycine: 0.00751 g/Liter
L-Histidine * HCl * H20: 0.02096 g/Liter
Hydroxy-L -proline: 0.01967 g/Liter
L-Isoleucine: 0.03936 g/Liter
L-Leucine: 0.03936 g/Liter
L-Lysine: 0.03654 g/Liter
L-Methionine: 0.01492 g/Liter
L-Phenylalanine: 0.01652 g/Liter
L-Proline: 0.01727 g/Liter
L-Serine: 0.02628 g/Liter
L-Threonine: 0.01787 g/Liter
L-Tryptophan: 0.00306 g/Liter
L-Tyrosine * 2Na * 2H20: 0.0261 g/Liter
L-Valine: 0.01757 g/Liter
Total: 11.9g/liter
L-Arginine * HCI: 0.04214 g/Liter
L-Asparagine * H20: 0.04503 g/Liter
L-Aspartic Acid: 0.01997 g/Liter
L Cysteine, Free base: 0.02424 g/Liter
L-Glutamic Acid, Sodium: 0.02207 g/Liter
L-Glutamine: 0.21915 g/Liter
Glycine: 0.00751 g/Liter
L-Histidine * HCl * H20: 0.02096 g/Liter
Hydroxy-L -proline: 0.01967 g/Liter
L-Isoleucine: 0.03936 g/Liter
L-Leucine: 0.03936 g/Liter
L-Lysine: 0.03654 g/Liter
L-Methionine: 0.01492 g/Liter
L-Phenylalanine: 0.01652 g/Liter
L-Proline: 0.01727 g/Liter
L-Serine: 0.02628 g/Liter
L-Threonine: 0.01787 g/Liter
L-Tryptophan: 0.00306 g/Liter
L-Tyrosine * 2Na * 2H20: 0.0261 g/Liter
L-Valine: 0.01757 g/Liter
Total: 11.9g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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Product Notes
The McCoy's 5A Medium w/L-Glutamine w/o Phenol Red (Catalog #AAA14813) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "McCoy's 5A Medium w/L-Glutamine w/o Phenol Red, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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