Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate Culture Media
Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Powder)
Calcium Chloride•2H2O: 0.165
Cupric Sulfate•5H2O: 0.0000025
Ferrous Sulfate•7H2O: 0.000834
Magnesium Chloride•6H2O: 0.09963
Magnesium Sulfate: 0.02528
Potassium Chloride: 0.305
Potassium Phosphate Mono: 0.06124
Sodium Chloride: 7.517
Sodium Phosphate Dibasic: 0.1324
Zinc Sulfate•7H2O: 0.000863
Amino Acids:
L-Alanine: 0.018
L-Arginine•HCl: 0.422
L-Asparagine•H2O: 0.03
L-Aspartic Acid: 0.026
L-Cysteine•HCL•H2O: 0.07026
L-Glutamic Acid: 0.03
L-Glutamine: 0.292
Glycine: 0.016
L-Histidine•HCl•H2O: 0.042
L-Isoleucine: 0.0078
L-Leucine: 0.0262
L-Lycine•HCl: 0.073
L-Methionine: 0.009
L-Phenylalanine: 0.01
L-Proline: 0.07
L-Serine: 0.021
L-Threonine: 0.0238
L-Tryptophan: 0.004
L-Tyrosine•2Na•2H2O: 0.01586
L-Valine: 0.0234
Vitamins:
L-Ascorbic Acid•Na: 0.15
D-Biotin: 0.0000073
Choline Chloride: 0.01396
Vitamin B-12: 0.00136
Folic Acid: 0.00132
myo-Inositol: 0.01802
Niacinamide: 0.00004
D-Pantothenic Acid, Ca: 0.000238
Pyridoxine•HCl: 0.00006
Riboflavin: 0.00004
Thiamine•HCl: 0.000337
Other:
Thymidine: 0.0007
D-Glucose: 1.802
Hypoxanthine: 0.00404
Linoleic Acid: 0.00009
Thioctic Acid: 0.000206
Phenol Red, Sodium: 0.00125
Putrescine•2HCl: 0.000161
Pyruvic Acid, Sodium: 0.22
Total: 11.75 g/liter
Ham's F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.
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Product Notes
The Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate (Catalog #AAA14814) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nutrient Mixture Ham's F-12 Coon's Modification w/L-Glutamine, Zinc Sulfate, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is hams F12 mixture?
Ham's F-12 is a basal medium specifically formulated by Kaighn for culturing mammalian cells, particularly epithelial cells and endothelial cells. It contains a balanced mixture of amino acids, vitamins, glucose, and inorganic salts optimized for cell growth in vitro.
What is the composition of F12?
Ham's F-12 contains 14 amino acids, 8 vitamins, 11 inorganic salts, glucose, sodium pyruvate, and trace elements. Common formulations include L-glutamine (2.5 mM) and phenol red. The medium is often combined with DMEM in 1:1 ratios for broader cell type compatibility.
How much is concentration of glucose in hams F12?
Standard Ham's F-12 contains approximately 1,200 mg/L (6.7 mM) of glucose. Some formulations are available as glucose-free variants for specialized applications. Verify the specific glucose concentration with your supplier's product datasheet.
What is Ham’s F-12 Nutrient Mix used for?
Ham's F-12 is used for culturing epithelial cells, endothelial cells, hepatocytes, and hybridomas. It's frequently combined with DMEM for broader applications. Common uses include cell line maintenance, primary cell culture, and therapeutic protein production in biopharmaceutical manufacturing.
Does this formulation contain L-glutamine or phenol red?
Standard Ham's F-12 typically contains both L-glutamine (2.5 mM) and phenol red. However, specialized formulations are available without these components - glutamine-free versions for sensitive applications and phenol red-free variants for fluorescence-based assays. Check product specifications for exact composition.
What cell types can be cultured with Ham’s F-12?
Ham's F-12 supports epithelial cells, endothelial cells, hepatocytes, fibroblasts, and hybridoma cell lines. When combined with DMEM (DMEM/F-12), it supports a broader range, including primary tissues and mammalian cells requiring enhanced amino acid profiles.
How long is the shelf life?
Ham's F-12 liquid formulations typically maintain stability for 12-18 months at 2-8°C when properly sealed. Powder formulations remain stable for 2-3 years at room temperature in original, unopened packaging. Always verify expiration dates on product labels and maintain appropriate storage conditions.
Is this product suitable for GMP or clinical use?
Ham's F-12 is available in GMP-grade formulations certified for therapeutic and clinical applications. These versions undergo rigorous quality testing, sterility assurance, and traceability documentation. Confirm GMP certification with your supplier, as standard laboratory-grade products are not suitable for clinical manufacturing.
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