RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin Culture Media
RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin (Powder)
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Calcium Nitrate•4H2O: 0.1 g/liter
Magnesium Sulfate: 0.04884 g/liter
Potassium Chloride: 0.4 g/liter
Sodium Chloride: 6 g/liter
Sodium Phosphate Dibasic: 0.8 g/liter
Amino Acids
L-Arginine: 0.2 g/liter
L-Asparagine (anhydrous): 0.05 g/liter
L-Aspartic Acid: 0.02 g/liter
L-Cystine•2HCl: 0.0652 g/liter
L-Glutamic Acid: 0.02 g/liter
L-Glutamine: 0.3 g/liter
Glycine: 0.01 g/liter
L-Histidine: 0.015 g/liter
Hydroxy-L-proline: 0.02 g/liter
L-Isoleucine: 0.05 g/liter
L-Leucine: 0.05 g/liter
L-Lysine•HCl: 0.04 g/liter
L-Methionine: 0.015 g/liter
L-Phenylalanine: 0.015 g/liter
L-Proline: 0.02 g/liter
L-Serine: 0.03 g/liter
L-Threonine: 0.02 g/liter
L-Tryptophan: 0.005 g/liter
L-Tyrosine•2Na•2H2O: 0.02883 g/liter
L-Valine: 0.02 g/liter
Vitamins
P-Amino Benzoic Acid: 0.001 g/liter
D-Biotin: Absent
Choline Chloride: 0.003 g/liter
Vitamin B-12: 0.000005 g/liter
Folic Acid: 0.001 g/liter
myo-Inositol: 0.035 g/liter
Niacinamide: 0.001 g/liter
D-Pantothenic Acid•Ca: 0.00025 g/liter
Pyridoxine•HCl: 0.001 g/liter
Riboflavin: 0.0002 g/liter
Thiamine•HCl: 0.001 g/liter
Other
D-Glucose: 2 g/liter
Glutathione: 0.001 g/liter
Phenol Red: Absent
Total: 10.4 g/Liter
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Product Notes
The RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin (Catalog #AAA14832) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is RPMI 1640 medium?
RPMI 1640 is a widely used mammalian cell culture medium developed at Roswell Park Memorial Institute. It supports the growth of lymphoid cell lines, primary lymphocytes, and many other suspension and adherent cell types used in research and biopharmaceutical applications.
What is the composition of RPMI media
RPMI 1640 contains amino acids, vitamins, inorganic salts, glucose, and a pH indicator (phenol red). Key components include 14 amino acids, 6 vitamins, essential minerals, and glucose at approximately 2,000 mg/L. The formulation is optimized for diverse mammalian cell culture requirements.
What are the ingredients in RPMI 1640 medium
Essential ingredients include L-glutamine, inorganic salts (potassium chloride, sodium phosphate), trace elements (zinc, copper, iron), vitamins (niacin, thiamine), amino acids (leucine, lysine, methionine), glucose, and sodium bicarbonate. Many commercial formulations also include HEPES buffer for pH stability.
How to make RPMI 1640 media
RPMI 1640 powder is dissolved in ultrapure water according to manufacturer instructions, typically requiring approximately 10.4 grams per liter. After dissolution, adjust pH to 7.2 using sodium bicarbonate or hydrochloric acid, filter-sterilize (0.22 μm), and store at 2-8°C. Aliquots can be frozen at -20°C for long-term storage.
Are RPMI and DMEM the same?
No, RPMI 1640 and DMEM are distinct media formulations. RPMI 1640 contains lower glucose (2,000 mg/L) and higher amino acid concentrations, making it ideal for lymphoid cells. DMEM contains higher glucose (5,500 mg/L) and fewer amino acids, better suited for fibroblasts and epithelial cells.
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