William's E Culture Media
William's E (with L- alanyl -glutamine)
Applications
Cell Culture
Synonyms
William's E; N/A; William's E (with L- alanyl -glutamine); William's E culture media
Form/Format
Liquid
Concentration
1x (varies by lot)
Applicable Applications for William's E culture media
Cell Culture
PH
7.2-7.4
D-glucose
2000mg/L
HEPES
Negative
L- Alanyl- L- glutamine
2mM
NaHCO3
2200mg/L
Phenol Red
10mg/L
Notes
- This product is for research use only.
- This product is sterilized by 0.1 um filtration.
- It is necessary to pay attention to the aseptic operation and avoid the contamination.
Sodium Pyruvate
25mg/L
Preparation and Storage
Store at 2-8 degree C, Shading light
Expiration Date: 12 months
Expiration Date: 12 months
Related Product Information for William's E culture media
William's E medium was designed by William and Gunn. It is mainly used for the long-term culture of rat liver epithelial cells, and can also be used for the culture of other mammalian liver cells. L-alanyl-L-glutamine is an advanced cell culture additive and as a substitute for L-glutamine. L-glutamine is a necessary nutrient element for cells. But it is unstable in solution and can be degraded spontaneously to form ammonia and pyroglutamic acid and ammonia is harmful to cells. L-alanyl-L-glutamine is stable in aqueous solution and does not degrade spontaneously. The mechanism used by the cell is that the cell gradually releases a peptidase into the culture medium during cell culture. The peptidase can hydrolyze L-alanyl-L-glutamine into L-alanine and L-glutamine. L-alanine and L-glutamine can be absorbed and utilized by cells. This not only increases the utilization rate of L-glutamine, but also does not produce excess ammonia, which is more beneficial to cell growth. William's medium contains many kinds of amino acids, vitamins, inorganic salts and other ingredients for cell culture, but does not contain protein, lipids or any growth factors. Therefore, the product should be used with serum or serum-free additives.
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Product Notes
The William's E (Catalog #AAA177759) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's William's E can be used in a range of immunoassay formats including, but not limited to, Cell Culture. Researchers should empirically determine the suitability of the William's E for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "William's E, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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