Standard Curve (Sample)
Guinea Pig Connexin 43 ELISA Kit | CX43 elisa kit
Guinea pig Connexin 43 ELISA Kit
Gene Names
cx43; gja1; cx43a1; zfCx43.3
Reactivity
Guinea Pig
Synonyms
Connexin 43; N/A; Guinea pig Connexin 43 ELISA Kit; CX43 elisa kit
Reactivity
Guinea Pig
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CX43 elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This CX43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig CX43. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CX43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX43 antibody and an CX43-HRP conjugate. The assay sample and buffer are incubated together with CX43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX43 concentration since CX43 from samples and CX43-HRP conjugate compete for the anti-CX43 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX43 from the sample, fewer sites are left to bind CX43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX43 concentration in each sample is interpolated from this standard curve.
INTENDED USE This CX43 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig CX43. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY CX43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX43 antibody and an CX43-HRP conjugate. The assay sample and buffer are incubated together with CX43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX43 concentration since CX43 from samples and CX43-HRP conjugate compete for the anti-CX43 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX43 from the sample, fewer sites are left to bind CX43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX43 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CX43 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,127 Da
NCBI Official Full Name
Cx43 protein
NCBI Official Synonym Full Names
connexin 43
NCBI Official Symbol
cx43
NCBI Official Synonym Symbols
gja1; cx43a1; zfCx43.3
NCBI Protein Information
gap junction alpha-1 protein; shf; sof; connexin-43; etID309742.9; short fin protein; gap junction protein, alpha 1
UniProt Protein Name
Gap junction alpha-1 protein
UniProt Gene Name
gja1
UniProt Synonym Gene Names
cx43; shf; sof; Cx43
UniProt Entry Name
CXA1_DANRE
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Product Notes
The Guinea Pig CX43 gja1 (Catalog #AAA82656) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA82656 ELISA Kit recognizes Guinea Pig CX43. It is sometimes possible for the material contained within the vial of "Connexin 43, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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