GRO-beta (CXCL2) Recombinant Protein | CXCL2 recombinant protein

Mouse GRO-beta (CXCL2) Recombinant

Cat. Num. AAA76112

• Purity: >95%, as determined by SDS-PAGE and HPLC
• Synonyms: GRO-beta (CXCL2); N/A; Mouse GRO-beta (CXCL2) Recombinant; Mouse GRO-b Recombinant; GRO-beta; GROg; cxcl2; mip2; mip-2; CXCL2 recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >95%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant GRO-beta/CXCL2 was lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 20mM Tris, pH 8.0.
• Sequence: MAPPTCRLLS AALVLLLLLA TNHQATGAVV ASELRCQCLK TLPRVDFKNI QSLSVTPPGP HCAQTEVIAT LKGGQKVCLD PEAPLVQKII QKILNKGKAN
• Sequence Length: 100

• Domain: IL8
• Host Note: Optimized DNA sequence encoding mouse GRO-beta CXCL2 mature chain was expressed in Escherichia Coli.
• Biological Activity: Determined by its ability to chemoattract transfected CXCR2 cells using a concentration range of 10-100 ng/ml.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
• Molecular Weight Note: Native mouse GRO-beta is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa.; ; Recombinant GRO-beta is a disulfide-linked homodimeric protein consisting of 74 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 1 year from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CXCL2 recombinant protein

Growth regulated oncogene- gamma belongs to the family of chemotyctic cytokines called chemokines. It is identical with MGSA (melanoma growth stimulatory activity) and the new designation is CXCL3. This factor is known mainly because of its chemotactic activity. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively. Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.

Product Categories/Family for CXCL2 recombinant protein

Cytokines

References

• cxcl1 regulates pulmonary host defense to klebsiella infection via CXCL2, cxcl5, nf-b, and mapks .J. Immunol., Nov 2010; 185: 6214 - 6225.
• identification of two novel chemokines (ccl2 and CXCL2) in b-chronic lymphocytic leukaemia (b-cll) and prolonged survival of primary b-cll cells in vitro .Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 3157.
• toll-like receptor 4 ligation on intrinsic renal cells contributes to the induction of antibody-mediated glomerulonephritis via cxcl1 and CXCL2 .J. Am. Soc. Nephrol., Jun 2007; 18: 1732 - 1739.
• selective local pmn recruitment by cxcl1 or CXCL2/3 injection does not cause inflammatory pain .J. Leukoc. Biol., May 2006; 79: 1022 - 1032.
• relative contribution of lix/cxcl5, kc/cxcl1, and mip–2/CXCL2 in lps keratitis .Invest. Ophthalmol. Vis. Sci., May 2006; 47: 4361.