Cyclin E Recombinant Protein | CCNE1 recombinant protein

Cyclin E Protein, Mouse, Recombinant (His & GST Tag)

Cat. Num. AAA258210

• Gene Names: Ccne1; CycE1; AW538188
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Cyclin E; N/A; Cyclin E Protein, Mouse, Recombinant (His & GST Tag); Mouse CCNE1/Cyclin-E1 Protein (His & GST Tag); AW538188 Protein, Mouse; CycE1 Protein, Mouse; cyclin E1; CCNE1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM Nacl, 3mM DTT, 0.5mM GSH, 10% gly, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of Co
• Sequence: Met1-Glu408

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: N-GST & His
• Protein Construction: A DNA sequence encoding the mouse CCNE1 (AAH62152.1)(Met1-Glu408) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured by its binding ability in a functional ELISA. Immobilized mouse CCNE1 at 10 ug/ml (100 ul/well) can bind biotinylated human CDK4, The EC50 of biotinylated human CDK4 is 48.0-114.0 ng/ml.)

SDS-PAGE

Related Product Information for CCNE1 recombinant protein

Background: Cyclin E1 is a member of the highly conserved cyclin family and belongs to the E-type cyclin that functions as a regulator of S phase entry and progression in mammalian cells. Cyclin E1 serves as regulatory subunits that bind, activate, and provide substrate for its associated cyclin-dependent kinase2 (CDK2), whose activity is essential for cell cycle G1/S transition. Over expression of this encoding gene has been found in many tumors, which results in chromosome instability and by extension, induce tumorigenesis. This protein was also found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB. In general, cyclin E1, as an activator of phospho-CDK2 (pCDK2), is important for cell cycle progression and is frequently overexpressed in cancer cells.

Product Categories/Family for CCNE1 recombinant protein

p53 Pathway

References

Honda R, et al. (2005) The structure of cyclin E1/CDK2: implications for CDK2 activation and CDK2-independent roles. The EMBO Journal. 24: 452-63.Geng Y, et al. (2007) Kinase-Independent Function of Cyclin E. 25(1): 127-39.

NCBI and Uniprot Product Information

• NCBI GI #: 116283967
• NCBI GeneID: 12447
• Molecular Weight: 46,986 Da
• NCBI Official Full Name: Cyclin E1
• NCBI Official Synonym Full Names: cyclin E1
• NCBI Official Symbol: Ccne1
• NCBI Official Synonym Symbols: CycE1; AW538188
• NCBI Protein Information: G1/S-specific cyclin-E1