IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-BCMA antibody CA8 . Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of CA8 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
BCMA Antibody | anti-BCMA antibody
Anti-BCMA [CA8]
Gene Names
CENPE; KIF10; CENP-E; PPP1R61
Reactivity
Human, Cynomolgus Monkey
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Protein A affinity purified
Synonyms
BCMA; N/A; Anti-BCMA [CA8]; CD269; BCM; TNFRSF17; Tumor necrosis factor receptor superfamily member 17; B-cell maturation protein; S307118G03; 118G03; anti-BCMA antibody
Reactivity
Human, Cynomolgus Monkey
Isotype
Mouse IgG1 kappa
Clone Number
CA8
Specificity
This antibody binds human and cynomolgus monkey BCMA and does not bind TACI or BAFF-R proteins. BCMA acts as a receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. BCMA expression is highest on terminally differentiated B cells. BCMA is involved in mediating the survival of plasma cells for maintaining long-term humoral immunity. It also activates NF-kappa-B and JNK. The expression of BCMA has been linked to a number of cancers, autoimmune disorders, and infectious diseases.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BCMA antibody
FCM/FACS (Flow Cytometry), ELISA
Immunogen
The original mouse parental antibody 118G03 was generated by immunizing SJL mice with recombinant human BCMA/TNFRSF17-Fc chimera (R&D 193-Fc) by using the RIMMS method (Rapid immunization multiple sites). A chimeric version of this antibody was generated and it is called CA8.
Preparation and Storage
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-BCMA antibody CA8 . Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of CA8 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of Daudi cells with anti-BCMA antibody CA8 . Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of CA8 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-BCMA antibody CA8 . Daudi cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of CA8 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow cytometry using anti-BCMA antibody CA8 . Daudi cells were stained with the anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of CA8 at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and the cells were analyzed using a FACSCanto flow-cytometer.)
Related Product Information for anti-BCMA antibody
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Product Categories/Family for anti-BCMA antibody
References
Yu-Tzu Tai et al.
Immunotherapy. 2015 Nov; 7(11): 1187-1199.
Immunotherapy. 2015 Nov; 7(11): 1187-1199.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
centromere-associated protein E
NCBI Official Synonym Full Names
centromere protein E, 312kDa
NCBI Official Symbol
CENPE
NCBI Official Synonym Symbols
KIF10; CENP-E; PPP1R61
NCBI Protein Information
centromere-associated protein E; kinesin family member 10; kinesin-related protein CENPE; Centromere autoantigen E (312kD); protein phosphatase 1, regulatory subunit 61
UniProt Protein Name
Centromere-associated protein E
UniProt Gene Name
CENPE
UniProt Synonym Gene Names
CENP-E
UniProt Entry Name
CENPE_HUMAN
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Product Notes
The BCMA cenpe (Catalog #AAA72354) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCMA [CA8] reacts with Human, Cynomolgus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's BCMA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the BCMA cenpe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCMA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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