WB (Western Blot)
(Western blot using anti-TREM-1 antibody 14F69 . Human spleen (A), bone marrow (B), and liver (C) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 14F69 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
TREM-1 Antibody | anti-TREM-1 antibody
Anti-TREM-1 [14F69]
Gene Names
TREM1; CD354; TREM-1
Reactivity
Human, Cynomolgus Monkey, Rhesus Monkey
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified. Protein A affinity purified
Synonyms
TREM-1; N/A; Anti-TREM-1 [14F69]; TREM1; CD354; Triggering receptor expressed on myeloid cells 1; Triggering receptor expressed on monocytes 1; anti-TREM-1 antibody
Reactivity
Human, Cynomolgus Monkey, Rhesus Monkey
Isotype
Mouse IgG2a kappa
Clone Number
14F69
Specificity
This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TREM-1 antibody
Blocking, FCM/FACS (Flow Cytometry), ELISA
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
WB (Western Blot)
(Western blot using anti-TREM-1 antibody 14F69 . Human spleen (A), bone marrow (B), and liver (C) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 14F69 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
WB (Western Blot)
(Western blot using anti-TREM-1 antibody 14F69 . Human spleen (A), bone marrow (B), and liver (C) tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of 14F69 at 0.1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.)
IF (Immunofluorescence)
(Immunofluorescence staining of K562 cells with anti-TREM-1 antibody 14F69 . Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14F69 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence staining of K562 cells with anti-TREM-1 antibody 14F69 . Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 14F69 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom,, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-TREM-1 antibody
The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.
Product Categories/Family for anti-TREM-1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,387 Da
NCBI Official Full Name
triggering receptor expressed on myeloid cells 1 isoform 2
NCBI Official Synonym Full Names
triggering receptor expressed on myeloid cells 1
NCBI Official Symbol
TREM1
NCBI Official Synonym Symbols
CD354; TREM-1
NCBI Protein Information
triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1; triggering receptor expressed on monocytes 1
UniProt Protein Name
Triggering receptor expressed on myeloid cells 1
UniProt Gene Name
TREM1
UniProt Synonym Gene Names
TREM-1
UniProt Entry Name
TREM1_HUMAN
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Product Notes
The TREM-1 trem1 (Catalog #AAA72343) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-TREM-1 [14F69] reacts with Human, Cynomolgus Monkey, Rhesus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's TREM-1 can be used in a range of immunoassay formats including, but not limited to, Blocking, FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the TREM-1 trem1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TREM-1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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