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product-image-AAA100101_SC13.jpg Standard Curve (Sample)

Human Cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit | CYP11A1 elisa kit

Human Cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit

Gene Names
CYP11A1; CYP11A; CYPXIA1; P450SCC
Reactivity
Human
Synonyms
Cholesterol side-chain cleavage enzyme, mitochondrial; N/A; Human Cholesterol side-chain cleavage enzyme, mitochondrial ELISA Kit; CYP11A1 (Cholesterol side-chain cleavage enzyme; mitochondrial)/CYP11A/Cytochrome P450 (scc)/Cytochrome P450 11A1/Cholesterol desmolase/; CYP11A1 elisa kit
Ordering
Reactivity
Human
Sequence Length
521
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
0.313-20ng/ml
Sensitivity
0.188ng/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

product-image-AAA100101_SC13.jpg Standard Curve (Sample)

Standard Curve (Sample)

product-image-AAA100101_SC15.jpg Standard Curve (Sample)
Related Product Information for CYP11A1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human CYP11A1antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human CYP11A1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CYP11A1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman CYP11A1can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,152 Da
NCBI Official Full Name
cholesterol side-chain cleavage enzyme, mitochondrial isoform a
NCBI Official Synonym Full Names
cytochrome P450 family 11 subfamily A member 1
NCBI Official Symbol
CYP11A1
NCBI Official Synonym Symbols
CYP11A; CYPXIA1; P450SCC
NCBI Protein Information
cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Protein Name
Cholesterol side-chain cleavage enzyme, mitochondrial
UniProt Gene Name
CYP11A1
UniProt Synonym Gene Names
CYP11A
UniProt Entry Name
CP11A_HUMAN

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Product Notes

The Human CYP11A1 cyp11a1 (Catalog #AAA100101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA100101 ELISA Kit recognizes Human CYP11A1. It is sometimes possible for the material contained within the vial of "Cholesterol side-chain cleavage enzyme, mitochondrial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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