Standard Curve (Sample)
Human Cytochrome P450 1B1 ELISA Kit | CYP1B1 elisa kit
Human Cytochrome P450 1B1 ELISA Kit
Gene Names
CYP1B1; CP1B; GLC3A; CYPIB1; P4501B1
Reactivity
Human
Synonyms
Cytochrome P450 1B1; N/A; Human Cytochrome P450 1B1 ELISA Kit; CYPIB1; CYP1B1; 1.14.14.1; CYP1B1 elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural Human Cytochrome P450 1B1. No significant cross-reactivity or interference was observed.
Sequence Length
543
Assay Type
Competitive
Detection Range
0.78-50 ng/mL
Sensitivity
0.407 ng/mL
Intra-Assay CV
<=7.8%
Inter-Assay CV
<=8.9%
Recovery
109%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CYP1B1 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Cytochrome P450 1B1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1B1, During the reaction, Cytochrome P450 1B1 in the sample or standard competes with a fixed amount of biotin-labeled Cytochrome P450 1B1 for sites on a pre-coated Monoclonal antibody specific to Cytochrome P450 1B1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cytochrome P450 1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 1B1, During the reaction, Cytochrome P450 1B1 in the sample or standard competes with a fixed amount of biotin-labeled Cytochrome P450 1B1 for sites on a pre-coated Monoclonal antibody specific to Cytochrome P450 1B1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Cytochrome P450 1B1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,846 Da
NCBI Official Full Name
cytochrome P450 1B1
NCBI Official Synonym Full Names
cytochrome P450 family 1 subfamily B member 1
NCBI Official Symbol
CYP1B1
NCBI Official Synonym Symbols
CP1B; GLC3A; CYPIB1; P4501B1
NCBI Protein Information
cytochrome P450 1B1
UniProt Protein Name
Cytochrome P450 1B1
UniProt Gene Name
CYP1B1
UniProt Entry Name
CP1B1_HUMAN
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Product Notes
The Human CYP1B1 cyp1b1 (Catalog #AAA195914) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA195914 ELISA Kit recognizes Human CYP1B1. It is sometimes possible for the material contained within the vial of "Cytochrome P450 1B1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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