Human Cytochrome P450 3A4 ELISA Kit | CYP3A4 elisa kit

Human Cytochrome P450 3A4 ELISA Kit

Cat. Num. AAA16518

• Gene Names: CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
• Reactivity: Human
• Synonyms: Cytochrome P450 3A4; Human Cytochrome P450 3A4 ELISA Kit; CYP3A4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CYP3A4. No significant cross-reactivity or interference between CYP3A4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CYP3A4 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive or Sandwich
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CYP3A4 elisa kit

Principle of the Assay: CYP3A4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP3A4. Standards or samples are then added to the microtiter plate wells and CYP3A4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP3A4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP3A4 are added to each well to "sandwich" the CYP3A4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP3A4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP3A4 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CYP3A4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CYP3A4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 322302351
• NCBI GeneID: 1576
• NCBI Accession #: NP_001189784.1
• NCBI GenBank Nucleotide #: NM_001202855.2
• Molecular Weight: 57,343 Da
• NCBI Official Full Name: cytochrome P450 3A4 isoform 2
• NCBI Official Synonym Full Names: cytochrome P450, family 3, subfamily A, polypeptide 4
• NCBI Official Symbol: CYP3A4
• NCBI Official Synonym Symbols: HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
• NCBI Protein Information: cytochrome P450 3A4; nifedipine oxidase; cytochrome P450 3A3; cytochrome P450 HLp; cytochrome P450-PCN1; cytochrome P450 NF-25; albendazole sulfoxidase; quinine 3-monooxygenase; albendazole monooxygenase; P450-III, steroid inducible; glucocorticoid-inducible P450; 1,8-cineole 2-exo-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4
• UniProt Protein Name: Cytochrome P450 3A4
• UniProt Gene Name: CYP3A4
• UniProt Synonym Gene Names: CYP3A3
• UniProt Entry Name: CP3A4_HUMAN