Porcine Cystatin C ELISA Kit | Cys-C elisa kit

Porcine Cystatin C ELISA Kit

Cat. Num. AAA17536

• Gene Names: CST3; ARMD11; HEL-S-2
• Reactivity: Porcine
• Synonyms: Cystatin C; Porcine Cystatin C ELISA Kit; Cys-C elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cys-C. No significant cross-reactivity or interference between Cys-C and analogues was observed.
• Sequence Length: 146
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cys-C were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cys-C were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for Cys-C elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Cys-C antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Cys-C antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Cys-C amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Cys-C can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 755738
• NCBI GeneID: 1471
• UniProt Accession #: P01034; Q6FGW9; B2R5J9; D3DW42
• Molecular Weight: 15,799 Da
• NCBI Official Full Name: cystatin C
• NCBI Official Synonym Full Names: cystatin C
• NCBI Official Symbol: CST3
• NCBI Official Synonym Symbols: ARMD11; HEL-S-2
• NCBI Protein Information: cystatin-C
• UniProt Protein Name: Cystatin-C
• UniProt Gene Name: CST3
• UniProt Entry Name: CYTC_HUMAN