Rat D.Dimer ELISA Kit | D.D elisa kit
Rat D.Dimer ELISA Kit
Reactivity
Rat
Synonyms
D.Dimer; N/A; Rat D.Dimer ELISA Kit; D.Dimer ELISA Kit (Rat); D.D elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of D2D. No significant cross-reactivity or interference between D2D and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between D2D and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ?g/mL
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Related Product Information for D.D elisa kit
Intended Uses: This D2D ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat D2D. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: D2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-D2D antibody and an D2D-HRP conjugate. The assay sample and buffer are incubated together with D2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D2D concentration since D2D from samples and D2D-HRP conjugate compete for the anti-D2D antibody binding site. Since the number of sites is limited, as more sites are occupied by D2D from the sample, fewer sites are left to bind D2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.
Principle of the Assay: D2D ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-D2D antibody and an D2D-HRP conjugate. The assay sample and buffer are incubated together with D2D-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D2D concentration since D2D from samples and D2D-HRP conjugate compete for the anti-D2D antibody binding site. Since the number of sites is limited, as more sites are occupied by D2D from the sample, fewer sites are left to bind D2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.
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