Mouse D-Dimer ELISA Kit | D2D elisa kit
Mouse D-Dimer ELISA Kit
Reactivity
Mouse
Synonyms
D-Dimer; N/A; Mouse D-Dimer ELISA Kit; D2D; D2D elisa kit
Reactivity
Mouse
Specificity
Specifically recognize D2D, no obvious cross reaction with other analogues
Assay Type
Sandwich
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
7.813-500ng/ml
Sensitivity
4.688ng/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for D2D elisa kit
Background: D-dimer is a fragment derived from the breakdown of fibrinogen in the process of blood clot dissolution in plasma. Under normal conditions, endothelial cells in blood vessels release an enzyme called tissue-type plasminogen activator (t-PA) to activate plasminogen and dissolve blood clots, ultimately forming D-dimer which is then metabolized by the liver. In certain pathological conditions, such as thrombotic disorders and deep vein thrombosis, the level of D-dimer will significantly increase, making it one of the indicators for diagnosing and monitoring these diseases. In addition, D-dimer may also increase in some infectious and inflammatory diseases, which may be related to inflammatory reactions and endothelial damage.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti D2D antibody was precoated onto the 96-well plate. The biotin conjugated anti D2D antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with D2D conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of D2D in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti D2D antibody was precoated onto the 96-well plate. The biotin conjugated anti D2D antibody was used as the detection antibody. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, biotinylated detection antibody was added to bind with D2D conjugated on coated antibody. After washing off unbound conjugates, HRP-Streptavidin was added. After a third washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of D2D in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.
