Human Dopamine Receptor D2 ELISA Kit | D2R/DRD2 elisa kit

Human Dopamine Receptor D2 ELISA Kit

Cat. Num. AAA17359

• Gene Names: DRD2; D2R; D2DR
• Reactivity: Human
• Synonyms: Dopamine Receptor D2; N/A; Human Dopamine Receptor D2 ELISA Kit; D2R/DRD2 (Dopamine Receptor D2); D2R/DRD2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of D2D. No significant cross-reactivity or interference between D2D and analogues was observed.
• Sequence Length: 443
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D2D were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D2D were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for D2R/DRD2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-D2D antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-D2D antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the D2D amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of D2D can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 5921480
• NCBI GeneID: 1813
• UniProt Accession #: P14416; Q9NZR3; Q9UPA9
• Molecular Weight: 50,847 Da
• NCBI Official Full Name: dopamine receptor D2
• NCBI Official Synonym Full Names: dopamine receptor D2
• NCBI Official Symbol: DRD2
• NCBI Official Synonym Symbols: D2R; D2DR
• NCBI Protein Information: D(2) dopamine receptor
• UniProt Protein Name: D(2) dopamine receptor
• UniProt Gene Name: DRD2
• UniProt Entry Name: DRD2_HUMAN