Rat Dopamine Transporter ELISA Kit | DAT elisa kit

Rat Dopamine Transporter ELISA Kit

Cat. Num. AAA16134

• Reactivity: Rat
• Synonyms: Dopamine Transporter; N/A; Rat Dopamine Transporter ELISA Kit; DAT elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DAT. No significant cross-reactivity or interference between DAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DAT and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for DAT elisa kit

Principle of the Assay: DAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DAT antibody and an DAT-HRP conjugate. The assay sample and buffer are incubated together with DAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DAT concentration since DAT from samples and DAT-HRP conjugate compete for the anti-DAT antibody binding site. Since the number of sites is limited, as more sites are occupied by DAT from the sample, fewer sites are left to bind DAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DAT concentration in each sample is interpolated from this standard curve.

Intended Uses: This DAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat DAT. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for DAT elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 223691974
• NCBI GeneID: 7501668
• Molecular Weight: 31,763 Da
• NCBI Official Full Name: Dat
• NCBI Official Symbol: dat
• NCBI Protein Information: D-alanine aminotransferase Dat; hypothetical protein
• UniProt Protein Name: Dat
• UniProt Gene Name: dat
• UniProt Synonym Gene Names: HRM2_21590
• UniProt Entry Name: C0QDJ3_DESAH