Human Decorin ELISA Kit | DCN elisa kit

Human Decorin PicoKine ELISA Kit

Cat. Num. AAA11571

• Gene Names: DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• Reactivity: Human
• Synonyms: Decorin; N/A; Human Decorin PicoKine ELISA Kit; decorin; Bone proteoglycan II; CSCD; DCN; DCN protein; Decorin proteoglycan; Dermatan sulphate proteoglycans II; DKFZp686J19238; DSPG 2; DSPG2; PG 40 ; PG II; PG S2; PG-S2; PG40; PGII; PGS 2; PGS2; PGS2_HUMAN; Proteoglycan core protein; SLRR1B; Small leucine rich protein 1B; DCN elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human DCN
• Sequence Length: 359
• Samples: Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <10 pg/ml

• Intra-assay Precision: Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

Related Product Information for DCN elisa kit

Background: Decorin is a proteoglycan on average 90 - 140 kilodaltons (kD) in size.It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a protein core containing leucine repeats with a glycosaminoglycan (GAG) chain consisting of either chondroitin sulfate (CS) or dermatan sulfate (DS).Using a genomic clone as the labeled probe and in situ hybridization of human metaphase chromosomes, decorin gene was mapped to the discrete region of human chromosome 12q23. Decorin is capable of suppressing the growth of various tumor cell lines when expressed ectopically.

Principle of the Assay: The Picokine™ Human DCN Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human DCN with a 96-well strip plate that is pre-coated with antibody specific for DCN. The detection antibody is a biotinylated antibody specific for DCN. The capture antibody is monoclonal antibody from mouse and the detection antibody is monoclonal antibody from mouse. The kit contains recombinant Human DCN with immunogen: Expression system for standard: sf21; Immunogen sequence: G17-K359. The kit is analytically validated with ready-to-use reagents. To measure Human DCN, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human DCN in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human DCN in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 129951
• NCBI GeneID: 1634
• UniProt Accession #: P07585; Q9P0Z0; Q9P0Z1; Q9Y5N8; Q9Y5N9
• Molecular Weight: 8,266 Da
• NCBI Official Full Name: Decorin
• NCBI Official Synonym Full Names: decorin
• NCBI Official Symbol: DCN
• NCBI Official Synonym Symbols: CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• NCBI Protein Information: decorin; PG-S2; bone proteoglycan II; decorin proteoglycan; proteoglycan core protein; small leucine-rich protein 1B; dermatan sulphate proteoglycans II
• UniProt Protein Name: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN