Standard Curve (Sample)
Human Defensin alpha 1 ELISA Kit | DEFA1 elisa kit
Human Defensin alpha 1 ELISA Kit
Gene Names
DEFA1B; HP1; HP-1; HNP-1
Reactivity
Human
Synonyms
Defensin alpha 1; N/A; Human Defensin alpha 1 ELISA Kit; DEF1; DEFA2; MRS; Neutrophil defensin 1; Defensin, alpha 1; HNP-1; HP-1; HP1; DEFA1 elisa kit
Reactivity
Human
Specificity
The Human Defensin alpha 1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Defensin alpha 1 protein within the range of 312 pg/ml - 20000 pg/ml.
Sequence Length
94
Samples
Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates
Sensitivity
< 150 pg/ml
Detection Method
Colorimetric
Preparation and Storage
Store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for DEFA1 elisa kit
Background: Human Defensin alpha 1 (DEFA1) is 94 amino acids (aa) in length and is cleaved into the following chains: HP 1-56 (aa 39-94), Neutrophil defensin 1 (aa 65-94) and Neutrophil defensin 2 (aa 66-94). Defensins are secreted peptides that kill microbes by permeabilizing their plasma membrane. DEFA1 has antimicrobial activity against Gram-negative and Gram-positive bacteria. ADP-ribosylation reduces cytotoxic and antibacterial activities, it is also known to enhance IL8 production.
Principle of the Assay: The Human Defensin alpha 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Defensin alpha 1 in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Human Defensin alpha 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Defensin alpha 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Defensin alpha 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Defensin alpha 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Principle of the Assay: The Human Defensin alpha 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Defensin alpha 1 in Cell Culture Supernates, Serum, Plasma, Cell Lysates, Tissue Homogenates. This assay employs an antibody specific for Human Defensin alpha 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Defensin alpha 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Defensin alpha 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Defensin alpha 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
10,201 Da
NCBI Official Full Name
neutrophil defensin 1 isoform 2 preproprotein
NCBI Official Synonym Full Names
defensin alpha 1B
NCBI Official Symbol
DEFA1B
NCBI Official Synonym Symbols
HP1; HP-1; HNP-1
NCBI Protein Information
neutrophil defensin 1
UniProt Protein Name
Neutrophil defensin 1
UniProt Gene Name
DEFA1
UniProt Synonym Gene Names
DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
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Product Notes
The Human DEFA1 defa1 (Catalog #AAA263321) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263321 ELISA Kit recognizes Human DEFA1. It is sometimes possible for the material contained within the vial of "Defensin alpha 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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