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Standard Curve (Sample)

Mouse Defensin Alpha 5, Paneth Cell Specific ELISA Kit | Defa5 elisa kit

Mouse Defensin Alpha 5, Paneth Cell Specific ELISA Kit

Gene Names
DEFA5; DEF5; HD-5
Reactivity
Mouse
Synonyms
Defensin Alpha 5; Paneth Cell Specific; Mouse Defensin Alpha 5; Paneth Cell Specific ELISA Kit; Defa5/Defensin-related cryptdin-5/; Defa5 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Defa5. No significant cross-reactivity or interference between Defa5 and analogues was observed.
Sequence Length
94
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Standard Curve (Sample)
Related Product Information for Defa5 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
10,071 Da
NCBI Official Full Name
defensin, alpha 5, Paneth cell-specific
NCBI Official Synonym Full Names
defensin alpha 5
NCBI Official Symbol
DEFA5
NCBI Official Synonym Symbols
DEF5; HD-5
NCBI Protein Information
defensin-5
UniProt Protein Name
Defensin-5
UniProt Gene Name
DEFA5
UniProt Synonym Gene Names
DEF5
UniProt Entry Name
DEF5_HUMAN

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Product Notes

The Mouse Defa5 defa5 (Catalog #AAA17683) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA17683 ELISA Kit recognizes Mouse Defa5. It is sometimes possible for the material contained within the vial of "Defensin Alpha 5, Paneth Cell Specific, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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