Mouse Beta-defensin 2 ELISA Kit | Defb2 elisa kit
Mouse Beta-defensin 2 ELISA Kit
Gene Names
DEFB4A; BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
Reactivity
Mouse
Synonyms
Beta-defensin 2; N/A; Mouse Beta-defensin 2 ELISA Kit; Defb2/BD-2/DEFB2/DEFB4A/DEFB4B/HBD-2/SAP-1/Beta-defensin 2/DEFB-2/DEFB2DEFB102/DEFB4beta-defensin 4A/Defensin; beta 2Skin-antimicrobial peptide 1/defensin; beta 4/defensin; beta 4A/HBD-2/hBD-2/SAP1BD-2; Defb2 elisa kit
Reactivity
Mouse
Specificity
Specifically recognize Defb2, no obvious cross reaction with other analogues
Sequence Length
64
Assay Type
Competitive
Samples
Serum, plasma, cell culture supernatant and other biological samples
Detection Range
62.5-4000pg/ml
Sensitivity
37.5pg/ml
Intra-assay Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Related Product Information for Defb2 elisa kit
Background: beta-defensin-2 (HbetaD-2) is a small cysteine-rich cationic skin-antimicrobial peptide (SAP) produced by a number of epithelial cells.beta-defensin-2 has antimicrobial activity against gram-negative bacteria and Candida, but not gram-positive Staphylococcus aureus. beta-defensin-2 can be used for the study of colitis.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Defb2. During the reaction, Defb2 in the sample or standard competes with a fixed amount of Defb2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Defb2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Defb2 in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Defb2. During the reaction, Defb2 in the sample or standard competes with a fixed amount of Defb2 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Defb2. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of Defb2 in the samples is then determined by comparing the OD of the samples to the standard curve. The concentration of the target substance was inversely proportional to the OD450 value.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
7,038 Da
NCBI Official Full Name
beta-defensin 2
NCBI Official Synonym Full Names
defensin beta 4A
NCBI Official Symbol
DEFB4A
NCBI Official Synonym Symbols
BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102
NCBI Protein Information
beta-defensin 4A
UniProt Protein Name
Beta-defensin 4A
UniProt Gene Name
DEFB4A
UniProt Synonym Gene Names
DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1
UniProt Entry Name
DFB4A_HUMAN
