Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human beta-defensins 2. No significant cross-reactivity or interference between human beta-defensins 2 and analogues was observed.
Sequence Length
64
Assay Type
Quantitative Sandwich
Samples
Serum, urine, cell culture supernates, tissue homogenates
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 15.6 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for DEFB4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
7,038 Da
NCBI Official Full Name
beta-defensin 4B
NCBI Official Synonym Full Names
defensin, beta 4B
NCBI Official Symbol
DEFB4B
NCBI Official Synonym Symbols
DEFB4P
NCBI Protein Information
beta-defensin 4B; defensin, beta 4, pseudogene
UniProt Protein Name
Beta-defensin 4A
UniProt Gene Name
DEFB4A
UniProt Synonym Gene Names
DEFB102; DEFB2; DEFB4
DEFB4B; BD-2; hBD-2; SAP1
DEFB4B; BD-2; hBD-2; SAP1
UniProt Entry Name
DFB4A_HUMAN
