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2-Step Plus Poly-HRP Detection Kit

2-Step Plus Poly-HRP Anti Rabbit/Mouse IgG Detection System (With DAB Solution)

Applications
Immunohistochemistry
Synonyms
2-Step Plus Poly-HRP; N/A; 2-Step Plus Poly-HRP Anti Rabbit/Mouse IgG Detection System (With DAB Solution); 2-Step Plus Poly-HRP detection kit
Ordering
Applicable Applications for 2-Step Plus Poly-HRP detection kit
IHC (Immunohistochemistry)
Sample dyeing
1. Dewax and hydrate the paraffin section.
2. Repair antigen of the tissue section according to special requirements of applied primary antibody.
3. Incubate with (3% H2O2)for 10min to eliminate endogenous peroxidase activity.
4. Wash with PBS or TBS, 2 min× 3 times.
5. Drain the PBS with absorbent paper, then add (Normal Goat Serum) to the section. Incubate for 30 min at 37°C.
6. Dry the liquid around the section with absorbent paper, and draw a circle around the tissue with an oily pen. Add primary antibody with proper dilution ratio, incubate at room temperature or 37°C for 1~2h or at 4°C overnight (then rewarm at 37°C for 30 min). Wash with PBS or TBS, 2 min ×3 times.
7. Add (Polymer Helper), incubate at room temperature or 37°Cfor 20min. Wash with PBS or TBS, 2 min ×3 times.
8. Add (Polyperoxidase-anti-Mouse/Rabbit IgG), incubate at room temperature or 37°Cfor 20~30min. Wash with PBS or TBS, 2 min ×3 times.
9. Add 1 drop (approximately 50 uL) of (DAB Concentrate) into each 1 mL of (DAB Substrate), mix fully and the mixed reagent is the DAB Working Solution. Prepare fresh solution before use and the prepared solution should be stored in the dark. Fresh prepared DAB Working Solution is valid within 4 hours and the unused solution must be abandoned.
10. Take control of the DAB coloration period, the color of tan or brownish yellow is the positive signal. Avoid of excessive reaction.
11. Wash the section with deionized water terminate the chromogenic reaction, then operate the procedures of counterstaining, dehydrating, transparentizing and sealing.
Preparation and Storage
Store at 2~8°C, shading light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening.
Related Product Information for 2-Step Plus Poly-HRP detection kit
Background: 2-step plus is a two-step immunohistochemical broad spectrum detection reagent. It polymerizes monovalent Fab fragments of secondary antibody and enzyme, which replaces the secondary antibody and tertiary antibody in traditional method, can directly amplify the binding signal of antibody-antigen. This method not only retains the specific binding ability of antibody with antigen, but also can effectively avoid space steric hindrance caused by excessive polymer molecules. Compared with the traditional SP three-step method, this kit has the characteristics of simple, rapid, high-sensitivity. This system abandons the using of biotin, so it can avoid background staining by endogenous biotin. It can be used in IHC, in which the primary antibody is monoclonal/polyclonal antibody derived from rabbit/mouse. The unique polymer auxiliary agent for macromolecular detection system can detect the binding primary antibody better. Diluent for DAB concentrated solution has been provided in this kit to avoid the influence of the different water acidity and alkalinity on the DAB Chromogenic Agent.

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Product Notes

The 2-Step Plus Poly-HRP (Catalog #AAA174691) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 2-Step Plus Poly-HRP can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the 2-Step Plus Poly-HRP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "2-Step Plus Poly-HRP, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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