Standard Curve (Sample)
Glycogen synthase (GCS) Detection Kit | GCS detection kit
Micro Glycogen synthase(GCS) Activity Assay Kit
Synonyms
Glycogen synthase (GCS); N/A; Micro Glycogen synthase(GCS) Activity Assay Kit; GCS detection kit
Samples
Animal Tissues, Cells or Bacteria
Features & Benefits
Determines glycogen synthase activity in animal tissues, cells, and bacteria.
Detailed methods for sample preparation and calculation of results are provided.
Detailed methods for sample preparation and calculation of results are provided.
Usage Notes
96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
Do not mix or substitute reagents or materials from other kit lots or vendors.
The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
The Glycogen Synthase Activity Assay Kit (micro assay) provides a simple method for the detection of glycogen synthase activity in biological samples such as animal tissues, cells and bacteria. In the assay, the GCS present in the sample catalyzes the formation of glycogen and UDP from UDPG and glucose residues, and pyruvate kinase and lactate dehydrogenase further catalyze the formation of NAD+ from NADH sequentially, and the rate of decrease of NADH is measured at 340 nm, which is indicative of GCS activity.
Do not mix or substitute reagents or materials from other kit lots or vendors.
The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
The Glycogen Synthase Activity Assay Kit (micro assay) provides a simple method for the detection of glycogen synthase activity in biological samples such as animal tissues, cells and bacteria. In the assay, the GCS present in the sample catalyzes the formation of glycogen and UDP from UDPG and glucose residues, and pyruvate kinase and lactate dehydrogenase further catalyze the formation of NAD+ from NADH sequentially, and the rate of decrease of NADH is measured at 340 nm, which is indicative of GCS activity.
Preparation and Storage
Storage at -20 degree C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for GCS detection kit
Principle of the Assay: Glycogen synthase (GCS) adds the glycosyl of UDPG to the original glycogen or the non-reducing end of glycogen protein, and connects them with alpha-1,4 glycosidic bonds. GCS is the rate-limiting enzyme in the process of glycogen synthesis in animals, and it is also the main target enzyme of insulin, which plays an important role in the process of glucose metabolism and maintaining the relative stability of blood sugar. GCS catalyzes UDPG and glucose residues to generate glycogen and UDP, and pyruvate kinase and lactate dehydrogenase further catalyze NADH to generate NAD+ in turn. The activity of GCS can be reflected by measuring the decline rate of NADH at 340 nm.
Product Categories/Family for GCS detection kit
Similar Products
Product Notes
The GCS (Catalog #AAA332639) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glycogen synthase (GCS), Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.