Luminescent Mycoplasma Detection Kit
Luminescent Mycoplasma Detection Kit
Synonyms
Luminescent Mycoplasma; N/A; Luminescent Mycoplasma Detection Kit; Mycoplasma; Mycoplasma PCR Detection; Luminescent Mycoplasma detection kit
Form/Format
Liquid solution
Samples
Cell
Assay Duration
15min
Kit Components
Mycoplasma Assay Buffer
Luciferase
Mycoplasma Substrate A
Mycoplasma Reagent B
Postive Control
Luciferase
Mycoplasma Substrate A
Mycoplasma Reagent B
Postive Control
Features & Benefits
Mycoplasma detection
Simple operation, fast and time-saving, the whole inspection process only two steps, only 15min.
Continuous and stable luminescence, accurate detection.
Simple operation, fast and time-saving, the whole inspection process only two steps, only 15min.
Continuous and stable luminescence, accurate detection.
Usage Notes
All reagents avoid repeated freezing and thawing, and it is recommended that they be used in separate containers, which must not be contaminated with ATP.
Preparation and Storage
Stored at -20 degree C for 12 months, protect from light
All reagents avoid repeated freezing and thawing, and it is recommended that they be used in separate containers, which must not be contaminated with ATP.
All reagents avoid repeated freezing and thawing, and it is recommended that they be used in separate containers, which must not be contaminated with ATP.
Related Product Information for Luminescent Mycoplasma detection kit
The Luminescent Mycoplasma Detection Kit utilizes Mycoplasma-specific kinase activity and detects it through a luminescent reaction catalyzed by ATP-dependent luciferase, and determines whether a sample is contaminated with Mycoplasma by comparing the change in ATP content before and after addition of the test reagent. When Mycoplasma is cleaved, it releases a unique kinase that reacts with the substrate and catalyzes the conversion of ADP to ATP.
Mycoplasma is the smallest and simplest prokaryote. It has the following characteristics: there is no cell wall structure, and many common antibiotics that target the cell wall, such as penicillin or beta-lactam antibiotics, are ineffective against mycoplasma; The size is between bacteria and viruses, about 0.2-0.8um, some mycoplasma can pass 0.22 um filter, conventional filtration is ineffective for mycoplasma; Due to their limited biosynthesis capacity, many mycoplasma are usually adsorbated or scattered on the cell surface and between cells, relying on the host for nutrition.
Mycoplasma pollution may seriously affect the state of cells, causing changes in gene expression and metabolic characteristics of cells, resulting in abnormal cell growth, differentiation and death, and seriously affecting cell function. At the same time, the reliability, repeatability and consistency of the experimental results are seriously affected, so the detection of mycoplasma contamination is very important.
Mycoplasma is the smallest and simplest prokaryote. It has the following characteristics: there is no cell wall structure, and many common antibiotics that target the cell wall, such as penicillin or beta-lactam antibiotics, are ineffective against mycoplasma; The size is between bacteria and viruses, about 0.2-0.8um, some mycoplasma can pass 0.22 um filter, conventional filtration is ineffective for mycoplasma; Due to their limited biosynthesis capacity, many mycoplasma are usually adsorbated or scattered on the cell surface and between cells, relying on the host for nutrition.
Mycoplasma pollution may seriously affect the state of cells, causing changes in gene expression and metabolic characteristics of cells, resulting in abnormal cell growth, differentiation and death, and seriously affecting cell function. At the same time, the reliability, repeatability and consistency of the experimental results are seriously affected, so the detection of mycoplasma contamination is very important.
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Product Notes
The Luminescent Mycoplasma (Catalog #AAA332629) is a Detection Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Luminescent Mycoplasma, Detection Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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