SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
DGKB recombinant protein
DGKB, Active
Applications
Kinase Assay
Synonyms
DGKB; N/A; DGKB, Active; DGK; DAGK2; DGK-BETA; DGKB recombinant protein
Host
Sf9 insect cells
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol.
Applicable Applications for DGKB recombinant protein
Kinase Assay
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for DGKB recombinant protein
Recombinant full-length human DGKB was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The DGKB gene accession number is NM_004080.
Diacylglycerol kinase beta (DAG kinase beta) is a member of class I diacylglycerol kinase family and is regulated by Ca+ (1). Via converting diacylglycerol into phosphatidic acid, diacylglycerol kinases decrease the diacylglycerol levels to regulate the activity of PKC, Rho and Ras families (2). DGK beta is localized in brain and is involved in emotion and long-term memory linked to cognitive function (3). Impaired levels of diacylglycerol by DGK beta limits the NADpHoxidase activation in phagosomes (4).
Diacylglycerol kinase beta (DAG kinase beta) is a member of class I diacylglycerol kinase family and is regulated by Ca+ (1). Via converting diacylglycerol into phosphatidic acid, diacylglycerol kinases decrease the diacylglycerol levels to regulate the activity of PKC, Rho and Ras families (2). DGK beta is localized in brain and is involved in emotion and long-term memory linked to cognitive function (3). Impaired levels of diacylglycerol by DGK beta limits the NADpHoxidase activation in phagosomes (4).
Product Categories/Family for DGKB recombinant protein
References
1. Merida, I. et al. Diacylglycerol kinases: at the hub of cell signaling. Biochem. J. (409), 1-18, 2008.
2. Kano, T. et al. Both the C1 domain and a basic amino acid cluster at the C-terminus are important for the neurite and branch induction ability of DGKb. Biochemical and Biophysical Research Communications. (447) 89-94, 2014.
3. Caricasole, A. et al. Molecular Cloning and Characterization of the Human Diacylglycerol Kinase beta (DGKb) Gene. The Journal of Biological Chemistry. (277) 4790-4796, 2002.
4. Schlam, D. et al. Diacylglycerol Kinases Terminate Diacylglycerol Signaling during the Respiratory Burst Leading to Heterogeneous Phagosomal NADpHOxidase Activation. The Journal of Biological Chemistry. (288), 23090-23104, 2013.
2. Kano, T. et al. Both the C1 domain and a basic amino acid cluster at the C-terminus are important for the neurite and branch induction ability of DGKb. Biochemical and Biophysical Research Communications. (447) 89-94, 2014.
3. Caricasole, A. et al. Molecular Cloning and Characterization of the Human Diacylglycerol Kinase beta (DGKb) Gene. The Journal of Biological Chemistry. (277) 4790-4796, 2002.
4. Schlam, D. et al. Diacylglycerol Kinases Terminate Diacylglycerol Signaling during the Respiratory Burst Leading to Heterogeneous Phagosomal NADpHOxidase Activation. The Journal of Biological Chemistry. (288), 23090-23104, 2013.
NCBI and Uniprot Product Information
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Product Notes
The DGKB (Catalog #AAA73409) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DGKB can be used in a range of immunoassay formats including, but not limited to, Kinase Assay. Researchers should empirically determine the suitability of the DGKB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DGKB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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