Standard Curve (Sample)
Rat Type I iodothyronine deiodinase ELISA Kit | Dio1 elisa kit
Rat Type I iodothyronine deiodinase ELISA Kit
Gene Names
Dio1; 5DI; ITDI1; TXDI1
Reactivity
Rat
Synonyms
Type I iodothyronine deiodinase; N/A; Rat Type I iodothyronine deiodinase ELISA Kit; Dio1/BYE1/C20orf158/DATF1/DIO1/BYE1/C20orf158/DATF-1/DATF1DIDO3/death associated transcription factor 1/death inducer-obliterator 1/Death-associated transcription factor 1/death-inducer obliterator 1/DIO1DIDO2/DIO-1hDido1/dJ885L7.8/DKFZp434P1115/FLJ11265/KIAA0333chromosome 20 open reading frame 158/MGC16140; Dio1 elisa kit
Reactivity
Rat
Sequence Length
257
Assay Type
Sandwich ELISA, Double Antibody
Samples
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Range
15.625-1000pg/ml
Sensitivity
9.375pg/ml
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Standard Curve (Sample)
Standard Curve (Sample)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for Dio1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Dio1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Dio1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Dio1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Dio1 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,730 Da
NCBI Official Full Name
type I iodothyronine deiodinase
NCBI Official Synonym Full Names
deiodinase, iodothyronine, type I
NCBI Official Symbol
Dio1
NCBI Official Synonym Symbols
5DI; ITDI1; TXDI1
NCBI Protein Information
type I iodothyronine deiodinase
UniProt Protein Name
Type I iodothyronine deiodinase
UniProt Gene Name
Dio1
UniProt Synonym Gene Names
Itdi1; Txdi1
UniProt Entry Name
IOD1_RAT
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Product Notes
The Rat Dio1 dio1 (Catalog #AAA99225) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA99225 ELISA Kit recognizes Rat Dio1. It is sometimes possible for the material contained within the vial of "Type I iodothyronine deiodinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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