Standard Curve (Sample)
Monkey Dystrophin ELISA Kit | DMD elisa kit
Monkey Dystrophin ELISA Kit
Gene Names
DMD; BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Reactivity
Monkey
Synonyms
Dystrophin; N/A; Monkey Dystrophin ELISA Kit; DMD elisa kit
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for DMD elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This DMD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey DMD. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY DMD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DMD antibody and an DMD-HRP conjugate. The assay sample and buffer are incubated together with DMD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DMD concentration since DMD from samples and DMD-HRP conjugate compete for the anti-DMD antibody binding site. Since the number of sites is limited, as more sites are occupied by DMD from the sample, fewer sites are left to bind DMD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DMD concentration in each sample is interpolated from this standard curve.
INTENDED USE This DMD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey DMD. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY DMD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DMD antibody and an DMD-HRP conjugate. The assay sample and buffer are incubated together with DMD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DMD concentration since DMD from samples and DMD-HRP conjugate compete for the anti-DMD antibody binding site. Since the number of sites is limited, as more sites are occupied by DMD from the sample, fewer sites are left to bind DMD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DMD concentration in each sample is interpolated from this standard curve.
Product Categories/Family for DMD elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
426,750 Da
NCBI Official Full Name
DMD
NCBI Official Synonym Full Names
dystrophin
NCBI Official Symbol
DMD
NCBI Official Synonym Symbols
BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
NCBI Protein Information
dystrophin
UniProt Protein Name
Dystrophin
UniProt Gene Name
DMD
UniProt Entry Name
DMD_HUMAN
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Product Notes
The Monkey DMD dmd (Catalog #AAA92765) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA92765 ELISA Kit recognizes Monkey DMD. It is sometimes possible for the material contained within the vial of "Dystrophin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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