Mouse Deoxyribonuclease gamma ELISA Kit | DNASE1L3 elisa kit
Mouse Deoxyribonuclease gamma (DNASE1L3) ELISA Kit
Gene Names
Dnase1l3; Lsd; Dhp2; DNasegamma
Reactivity
Mouse
Synonyms
Deoxyribonuclease gamma; N/A; Mouse Deoxyribonuclease gamma (DNASE1L3) ELISA Kit; DHP2; DNAS1L3; LSD; DNase I homolog protein 2|DNase gamma|LS-DNase|Liver and spleen DNase|deoxyribonuclease I-like III|deoxyribonuclease gamma; DNASE1L3 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse DNASE1L3. No significant cross-reactivity or interference between Mouse DNASE1L3 and analogues was observed.
Sequence Length
275
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for DNASE1L3 elisa kit
DNASE1L3 encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
The predicted 306-amino acid protein was 45% identical to that of DNASE1. Northern blot analysis showed that DNASE1L3 was expressed primarily in spleen and liver cells. The predominant mRNA was 1.3 kb, although there were 2 other transcripts. The purified recombinant protein had DNase activity in vitro that, unlike that of DNASE1, was not inhibited by G-actin.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DNASE1L3 in samples. An antibody specific for DNASE1L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNASE1L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
The predicted 306-amino acid protein was 45% identical to that of DNASE1. Northern blot analysis showed that DNASE1L3 was expressed primarily in spleen and liver cells. The predominant mRNA was 1.3 kb, although there were 2 other transcripts. The purified recombinant protein had DNase activity in vitro that, unlike that of DNASE1, was not inhibited by G-actin.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DNASE1L3 in samples. An antibody specific for DNASE1L3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNASE1L3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNASE1L3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNASE1L3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,760 Da
NCBI Official Full Name
deoxyribonuclease gamma
NCBI Official Synonym Full Names
deoxyribonuclease 1-like 3
NCBI Official Symbol
Dnase1l3
NCBI Official Synonym Symbols
Lsd; Dhp2; DNasegamma
NCBI Protein Information
deoxyribonuclease gamma
UniProt Protein Name
Deoxyribonuclease gamma
UniProt Gene Name
Dnase1l3
UniProt Synonym Gene Names
DNase gamma; DNase I-like 3; LS-DNase; LSD
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Product Notes
The Mouse DNASE1L3 dnase1l3 (Catalog #AAA55882) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA55882 ELISA Kit recognizes Mouse DNASE1L3. It is sometimes possible for the material contained within the vial of "Deoxyribonuclease gamma, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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