Application Data
(The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 uM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 uM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent AAA283374, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data))
DNMT1 recombinant protein
Recombinant Human DNMT1 Protein
Synonyms
DNMT1; N/A; Recombinant Human DNMT1 Protein; DNMT1, AIM, CXXC9, DNMT, DNA (cytosine-5)-methyltransferase 1, Dnmt1, 2.1.1.37, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT; DNMT1 recombinant protein
Host
HEK293 cells
Form/Format
Supplied as 0.22 um filtered solution in 25mM Hepes-NaOH, 500mM NaCl, 0.04%, tritonX 100, 0.5mM TCEP, 10%glycerol, pH7.5.
Sequence
PARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKRSRDPPASASQVTGIRAEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD
Species
Human
Tag
C-His
Endotoxin
<1EU/ug
Bio-Activity
The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 uM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 uM SAM in reaction buffer (20 mM Tris-HCl [pH8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 degree C for 1 hr. Then methyltransferase assay was performed using MTase-Glo Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent RP01868LQ, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data)
Preparation and Storage
Store at -70 degree C. This product is stable at <= -70 degree C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
Application Data
(The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 uM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 uM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent AAA283374, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data))
Application Data
(The 42 base pair of DNA duplex containing three hemimethylation sites (2.5 uM) was methylated with the recombinant DNMT1 (0.18 mg/mL) including 20 uM SAM in reaction buffer (20 mM Tris-HCl [pH 8.0], 50 mM NaCl, 1 mM EDTA, 3 mM MgCl2, 0.1 mg/mL BSA, and 1 mM DTT) at 37 °C for 1 hr. Then methyltransferase assay was performed using MTase-Glo™ Methyltransferase Assay (Promega, V7601). Enzyme 1-4 represent AAA283374, Enzyme 5-8 represent recombinant DNMT1 with a 6×His tag at the N-terminus. (Customer feedback data))
SDS-PAGE
(Recombinant Human DNMT1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 250-300 kDa.)
SDS-PAGE
(Recombinant Human DNMT1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 250-300 kDa.)
Related Product Information for DNMT1 recombinant protein
DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Product Categories/Family for DNMT1 recombinant protein
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DNMT1 dnmt1 (Catalog #AAA283374) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: PARTAPARVP TLAVPAISLP DDVRRRLKDL ERDSLTEKEC VKEKLNLLHE FLQTEIKNQL CDLETKLRKE ELSEEGYLAK VKSLLNKDLS LENGAHAYNR EVNGRLENGN QARSEARRVG MADANSPPKP LSKPRTPRRS KSDGEAKRSR DPPASASQVT GIRAEPSPSP RITRKSTRQT TITSHFAKGP AKRKPQEESE RAKSDESIKE EDKDQDEKRR RVTSRERVAR PLPAEEPERA KSGTRTEKEE ERDEKEEKRL RSQTKEPTPK QKLKEEPDRE ARAGVQADED EDGDEKDEKK HRSQPKDLAA KRRPEEKEPE KVNPQISDEK DEDEKEEKRR KTTPKEPTEK KMARAKTVMN SKTHPPKCIQ CGQYLDDPDL KYGQHPPDAV DEPQMLTNEK LSIFDANESG FESYEALPQH KLTCFSVYCK HGHLCPIDTG LIEKNIELFF SGSAKPIYDD DPSLEGGVNG KNLGPINEWW ITGFDGGEKA LIGFSTSFAE YILMDPSPEY APIFGLMQEK IYISKIVVEF LQSNSDSTYE DLINKIETTV PPSGLNLNRF TEDSLLRHAQ FVVEQVESYD EAGDSDEQPI FLTPCMRDLI KLAGVTLGQR RAQARRQTIR HSTREKDRGP TKATTTKLVY QIFDTFFAEQ IEKDDREDKE NAFKRRRCGV CEVCQQPECG KCKACKDMVK FGGSGRSKQA CQERRCPNMA MKEADDDEEV DDNIPEMPSP KKMHQGKKKK QNKNRISWVG EAVKTDGKKS YYKKVCIDAE TLEVGDCVSV IPDDSSKPLY LARVTALWED SSNGQMFHAH WFCAGTDTVL GATSDPLELF LVDECEDMQL SYIHSKVKVI YKAPSENWAM EGGMDPESLL EGDDGKTYFY QLWYDQDYAR FESPPKTQPT EDNKFKFCVS CARLAEMRQK EIPRVLEQLE DLDSRVLYYS ATKNGILYRV GDGVYLPPEA FTFNIKLSSP VKRPRKEPVD EDLYPEHYRK YSDYIKGSNL DAPEPYRIGR IKEIFCPKKS NGRPNETDIK IRVNKFYRPE NTHKSTPASY HADINLLYWS DEEAVVDFKA VQGRCTVEYG EDLPECVQVY SMGGPNRFYF LEAYNAKSKS FEDPPNHARS PGNKGKGKGK GKGKPKSQAC EPSEPEIEIK LPKLRTLDVF SGCGGLSEGF HQAGISDTLW AIEMWDPAAQ AFRLNNPGST VFTEDCNILL KLVMAGETTN SRGQRLPQKG DVEMLCGGPP CQGFSGMNRF NSRTYSKFKN SLVVSFLSYC DYYRPRFFLL ENVRNFVSFK RSMVLKLTLR CLVRMGYQCT FGVLQAGQYG VAQTRRRAII LAAAPGEKLP LFPEPLHVFA PRACQLSVVV DDKKFVSNIT RLSSGPFRTI TVRDTMSDLP EVRNGASALE ISYNGEPQSW FQRQLRGAQY QPILRDHICK DMSALVAARM RHIPLAPGSD WRDLPNIEVR LSDGTMARKL RYTHHDRKNG RSSSGALRGV CSCVEAGKAC DPAARQFNTL IPWCLPHTGN RHNHWAGLYG RLEWDGFFST TVTNPEPMGK QGRVLHPEQH RVVSVRECAR SQGFPDTYRL FGNILDKHRQ VGNAVPPPLA KAIGLEIKLC MLAKARESAS AKIKEEEAAK D. It is sometimes possible for the material contained within the vial of "DNMT1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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