Mouse Deoxycorticosterone ELISA Kit | DOC elisa kit
Mouse Deoxycorticosterone ELISA Kit
Reactivity
Mouse
Synonyms
Deoxycorticosterone; N/A; Mouse Deoxycorticosterone ELISA Kit; DOC elisa kit
Reactivity
Mouse
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for DOC elisa kit
Intended Uses: This D-CORT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey D-CORT. This ELISA kit for research use only!
Principle of the Assay: D-CORT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-D-CORT antibody and an D-CORT-HRP conjugate. The assay sample and buffer are incubated together with D-CORT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D-CORT concentration since D-CORT from samples and D-CORT-HRP conjugate compete for the anti-D-CORT antibody binding site. Since the number of sites is limited, as more sites are occupied by D-CORT from the sample, fewer sites are left to bind D-CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D-CORT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: D-CORT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-D-CORT antibody and an D-CORT-HRP conjugate. The assay sample and buffer are incubated together with D-CORT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the D-CORT concentration since D-CORT from samples and D-CORT-HRP conjugate compete for the anti-D-CORT antibody binding site. Since the number of sites is limited, as more sites are occupied by D-CORT from the sample, fewer sites are left to bind D-CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D-CORT concentration in each sample is interpolated from this standard curve.
