anti-Human, Dog CD132 Antibody | anti-CD132 antibody

Anti-Hu CD132 APC

Cat. Num. AAA129305

• Gene Names: IL2RG; P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
• Reactivity: Human, Dog
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD132; N/A; Anti-Hu CD132 APC; interleukin 2 receptor subunit gamma; common gamma chain; IL2RG; SCIDX; IMD4; anti-CD132 antibody

• Reactivity: Human, Dog
• Isotype: Rat IgG2b kappa
• Clone Number: TUGh4
• Specificity: The rat monoclonal antibody TUGh4 recognizes an extracellular epitope of CD132 (the common gamma chain), a 65-70 kDa type I transmembrane glycoprotein broadly expressed by most leukocytes.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-CD132 antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Human CD132-transfected cell line
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD132 (TUGh4) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human CD132 (TUGh4) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD132 (TUGh4) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-CD132 antibody

CD132/common gamma chain is an essential component of receptors for IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, and it is critical for development of the immune system. Its mutation causes X-linked severe combined immunodeficiency disease (XSCID). CD132 is expressed on lymphocytes, NK cells, monocytes, and granulocytes. Through its cytoplasmic part which containsfour tyrosines and an SH2 domain, CD132 transcuces signal to downstream JAK/STAT pathway.

References

*Corrigall VM, Arastu M, Khan S, Shah C, Fife M, Smeets T, Tak PP, Panayi GS: Functional IL-2 receptor beta (CD122) and gamma (CD132) chains are expressed by fibroblast-like synoviocytes: activation by IL-2 stimulates monocyte chemoattractant protein-1 production. J Immunol. 2001 Mar 15;166(6):4141-7., URL: https://pubmed.ncbi.nlm.nih.gov/11238664/,*Brisslert M, Bokarewa M, Larsson P, Wing K, Collins LV, Tarkowski A: Phenotypic and functional characterization of human CD25+ B cells. Immunology. 2006 Apr;117(4):548-57., URL: http://www.ncbi.nlm.nih.gov/pubmed/16556269,*Amu S, Brisslert M: Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin Dev Immunol. 2011;2011:481948., URL: http://www.ncbi.nlm.nih.gov/pubmed/21941578,*Fiorito S, Magrini L, Adrey J, Mailhé D, Brouty-Boyé D: Inflammatory status and cartilage regenerative potential of synovial fibroblasts from patients with osteoarthritis and chondropathy. Rheumatology (Oxford). 2005 Feb;44(2):164-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/15479753,*Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK: Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33., URL: http://www.ncbi.nlm.nih.gov/pubmed/20074604,*Itano M, Tsuchiya S, Morita S, Fujie H, Ishii N, Yanagisawa T, Ohashi Y, Minegishi M, Sugamura K, Konno T: IL-2 receptor gamma chain expression on CD34 positive hematopoietic progenitor cells from bone marrow and cord blood. Tohoku J Exp Med. 1996 Apr;178(4):389-98., URL: http://www.ncbi.nlm.nih.gov/pubmed/8804156,

NCBI and Uniprot Product Information

• NCBI GI #: 400048
• NCBI GeneID: 3561
• UniProt Accession #: P31785; Q5FC12
• Molecular Weight: 369
• NCBI Official Full Name: Cytokine receptor common subunit gamma
• NCBI Official Synonym Full Names: interleukin 2 receptor, gamma
• NCBI Official Symbol: IL2RG
• NCBI Official Synonym Symbols: P64; CIDX; IMD4; CD132; SCIDX; IL-2RG; SCIDX1
• NCBI Protein Information: cytokine receptor common subunit gamma; gammaC; CD132 antigen; IL-2R subunit gamma; IL-2 receptor subunit gamma; common cytokine receptor gamma chain
• UniProt Protein Name: Cytokine receptor common subunit gamma
• UniProt Gene Name: IL2RG
• UniProt Synonym Gene Names: IL-2 receptor subunit gamma; IL-2R subunit gamma; IL-2RG
• UniProt Entry Name: IL2RG_HUMAN