WB (Western Blot)
(AAA61060 (0.5ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
OCT4/POU5F1 Antibody | anti-POU5F1 antibody
Goat anti-OCT4/POU5F1 Antibody
Gene Names
POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Reactivity
Tested: Human, MouseExpected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Western Blot, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
OCT4/POU5F1; N/A; Goat anti-OCT4/POU5F1 Antibody; POU5F1; OTF4; POU domain, class 5, transcription factor 1; HGNC: 9221; MGC22487; OCT3; OTF3; POU-type homeodomain-containing DNA-binding protein; octamer-binding transcription factor-3; POU class 5 homeobox 1; DADB-104B20.2; OCT4; POU domain, class 5, tran; POU5F1 antibody; OTF4 antibody; POU domain; class 5; transcription factor 1 antibody; HGNC: 9221 antibody; MGC22487 antibody; OCT3 antibody; OTF3 antibody; POU-type homeodomain-containing DNA-binding protein antibody; octamer-binding transcription factor-3 antibody; POU class 5 homeobox 1 antibody; DADB-104B20.2 antibody; OCT4 antibody; OCT4 / POU5F1; anti-POU5F1 antibody
Reactivity
Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence
VTTLGSPMHSN
Sequence Length
360
Applicable Applications for anti-POU5F1 antibody
WB (Western Blot), ELISA (Peptide ELISA)
Immunogen
Peptide with sequence C-VTTLGSPMHSN, from the C Terminus of the protein sequence according to NP_002692.2; NP_976034.3.
Note
This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8628
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
WB (Western Blot)
(AAA61060 (0.5ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(AAA61060 (0.5ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
(AAA61060 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
WB (Western Blot)
(AAA61060 (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
POU domain, class 5, transcription factor 1 isoform 1
NCBI Official Synonym Full Names
POU class 5 homeobox 1
NCBI Official Symbol
POU5F1
NCBI Official Synonym Symbols
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
NCBI Protein Information
POU domain, class 5, transcription factor 1
UniProt Protein Name
POU domain, class 5, transcription factor 1
UniProt Gene Name
POU5F1
UniProt Synonym Gene Names
OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
UniProt Entry Name
PO5F1_HUMAN
Similar Products
Product Notes
The POU5F1 pou5f1 (Catalog #AAA61060) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-OCT4/POU5F1 Antibody reacts with Tested: Human, Mouse Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's OCT4/POU5F1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA (Peptide ELISA). Researchers should empirically determine the suitability of the POU5F1 pou5f1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: VTTLGSPMHS N. It is sometimes possible for the material contained within the vial of "OCT4/POU5F1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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