IEP (Immunoelectrophoresis)
IgG (H&L), F(ab)'2 fragment Secondary Antibody
Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, Biotin Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, ELISA
Purity
Antibody was prepared using solid phase Mouse IgG.F(ab)'2 fragment was prepared by pepsin digestion.
Affinity purified is >90% based on SDS-PAGE
Synonyms
IgG (H&L), F(ab)'2 fragment; N/A; Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, Biotin Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG; Donkey anti-mouse IgG (H&L), F(ab)'2 fragment; IgG (H&L), F(ab)'2 fragment secondary antibody
Host
Donkey
Reactivity
Mouse
Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on mouse IgG, light chains on all mouse immunoglobulins
Purity/Purification
Antibody was prepared using solid phase Mouse IgG.
F(ab)'2 fragment was prepared by pepsin digestion.
Affinity purified is >90% based on SDS-PAGE
F(ab)'2 fragment was prepared by pepsin digestion.
Affinity purified is >90% based on SDS-PAGE
Form/Format
Clear, colorless liquid
Concentration
1.5 mg/ml (E1% at 280 nm = 13.0) (varies by lot)
Applicable Applications for IgG (H&L), F(ab)'2 fragment secondary antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Conjugate
Biotin
Immunogen
Purified Mouse IgG, whole molecule
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% (w/v) Sodium Azide
Cross Reactivity
Based on IEP, no reactivity is observed to:
non-immunoglobulin mouse serum immunoglobulins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
non-immunoglobulin mouse serum immunoglobulins
IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep
Preparation and Storage
Store undiluted liquid at 2-8 degree C.
Prepare working dilution prior to use and then discard.
Prepare working dilution prior to use and then discard.
IEP (Immunoelectrophoresis)
IEP (Immunoelectrophoresis)
SDS-PAGE
SDS-PAGE
Application Data
Application Data
Product Categories/Family for IgG (H&L), F(ab)'2 fragment secondary antibody
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Product Notes
The IgG (H&L), F(ab)'2 fragment (Catalog #AAA79070) is a Secondary Antibody produced from Donkey and is intended for research purposes only. The product is available for immediate purchase. The Donkey anti-Mouse IgG (H&L) - F(ab)'2 fragment, Biotin Conjugate, min x w/bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit, rat or sheep IgG reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (H&L), F(ab)'2 fragment can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the IgG (H&L), F(ab)'2 fragment for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (H&L), F(ab)'2 fragment, Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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