Mouse Dentin sialophosphoprotein ELISA Kit | DSPP elisa kit

Mouse Dentin sialophosphoprotein (DSPP) ELISA Kit

Cat. Num. AAA55932

• Gene Names: Dspp; Dpp; Dsp; Dmp2; Dmp3
• Reactivity: Mouse
• Synonyms: Dentin sialophosphoprotein; N/A; Mouse Dentin sialophosphoprotein (DSPP) ELISA Kit; DFNA39; DGI1; DMP3; DPP; DSP; DTDP2; dentin phosphophoryn|dentin phosphoprotein|dentin phosphoryn|dentin sialoprotein; DSPP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse DSPP. No significant cross-reactivity or interference between Mouse DSPP and analogues was observed.
• Sequence Length: 1301
• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich

• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for DSPP elisa kit

Dentin sialophosphoprotein, is the only protein produced uniquely by odontoblasts, the cells that produce tooth dentin. It is a non-collagenous SIBLING protein that is later cleaved into three functional proteins: dentin phosphoprotein (also known as phosphophoryn), taken from the C-terminal end, dentin sialoprotein from the N-terminal end, and dentin glycoprotein from the middle of the molecule. Their distribution in the collagen matrix of the forming dentin suggests these proteins play an important role in the regulation of mineral deposition. Additional evidence for this correlation is phenotypically manifested in patients with mutant forms of dentin sialophosphoprotein. Such patients suffer dental anomalies including type III dentinogenesis imperfecta.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate DSPP in samples. An antibody specific for DSPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDSPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DSPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DSPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 17865460
• NCBI GeneID: 666279
• UniProt Accession #: P97399; O70567
• Molecular Weight: 93,903 Da
• NCBI Official Full Name: Dentin sialophosphoprotein
• NCBI Official Synonym Full Names: dentin sialophosphoprotein
• NCBI Official Symbol: Dspp
• NCBI Official Synonym Symbols: Dpp; Dsp; Dmp2; Dmp3
• NCBI Protein Information: dentin sialophosphoprotein
• UniProt Protein Name: Dentin sialophosphoprotein
• UniProt Gene Name: Dspp
• UniProt Synonym Gene Names: Dmp3; DMP-3; DPP; DSP