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Application Data
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) Active Protein | ALDH1A1 active protein
Active Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1)
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Applications
Activity Assay, Cell Culture
Purity
>95%
Synonyms
Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1); N/A; Active Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1); ALDH11; ALDH1; ALDC; PUMB1; RALDH1; Retinaldehyde Dehydrogenase 1; Aldehyde dehydrogenase, cytosolic; Retinal dehydrogenase 1; ALDH1A1 active protein
Host
E. coli
Purity/Purification
>95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5 %Trehalose.
Concentration
200ug/mL (varies by lot)
Applicable Applications for ALDH1A1 active protein
Activity Assay, Cell Culture
Source
Prokaryotic expression.
Residues
Ser2~Ser501
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
6.3
Reconstitution
Reconstitute in ddH2O to a concentration <0.1mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
Application Data
WB (Western Blot)
(Sample: Recombinant ALDH1A1, Human;Antibody: Rabbit Anti-Human ALDH1A1 Ab)
WB (Western Blot)
(Sample: Recombinant ALDH1A1, Human;Antibody: Rabbit Anti-Human ALDH1A1 Ab)
SDS-PAGE
(Sample: Active recombinant ALDH1A1, Human)
SDS-PAGE
(Sample: Active recombinant ALDH1A1, Human)
Bioactivity
(ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner.)
Bioactivity
(ALDH1A1 (Retinal dehydrogenase 1) which belongs to the aldehyde dehydrogenase family, is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. ALDH1A1 converts retinaldehyde to retinoic acid. IL21 (interleukin 21) was identified as an interactor of ALDH1A1 through Affinity Capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human ALDH1A1 and recombinant human IL21. Briefly, ALDH1A1 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL ALDH1A1 were then transferred to IL21-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-ALDH1A1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ALDH1A1 and IL21 was shown in Figure 1, and this effect was in a dose dependent manner.)
Sequence
Sequence
Product Categories/Family for ALDH1A1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Mass: 58.4kDa
Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 58kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
retinal dehydrogenase 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family member A1
NCBI Official Symbol
ALDH1A1
NCBI Official Synonym Symbols
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
NCBI Protein Information
retinal dehydrogenase 1
UniProt Protein Name
Retinal dehydrogenase 1
UniProt Gene Name
ALDH1A1
UniProt Synonym Gene Names
RALDH 1Curated; RalDH1Curated
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Product Notes
The ALDH1A1 aldh1a1 (Catalog #AAA148210) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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