Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF) Active Protein | GMCSF active protein

Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF)

Cat. Num. AAA146599

• Gene Names: Csf2; Csfgm; GMCSF; Gm-CSf; MGI-IGM
• Applications: Activity Assay, Cell Culture
• Purity: > 90%
• Synonyms: Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF); N/A; Active Colony Stimulating Factor 2, Granulocyte Macrophage (GMCSF); CSF2; GM-CSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor; GMCSF active protein

• Host: E Coli
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 141

• Applicable Applications for GMCSF active protein: Activity Assay, Cell Culture
• Fragment: Pro19~Lys141
• Tag: N-terminal His and GST Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Organism Species: Mouse
• Isoelectric Point: 5.9
• Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Application Data

SDS-PAGE

Activity

(Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED50 (median effective dose) for this effect is less than 0.25 ng/mL.In-house data of APA045Mu01 used in cellular experiment:Six-eight weeks old Balb/c mice were used for BMDC. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI 1640 medium, followed by centrifugation at 1200RPM for 5min (4oC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4oC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and GMCSF (APA045Mu01) at 37oC with 5% CO2 in thermostatic incubator. Three days later, cells were observed by microscope, and the result is shown in Figure 1.)

Product Categories/Family for GMCSF active protein

Active Protein

NCBI and Uniprot Product Information

• NCBI GI #: 110625658
• NCBI GeneID: 12981
• NCBI Accession #: NP_034099.2
• NCBI GenBank Nucleotide #: NM_009969.4
• UniProt Accession #: P01587
• Molecular Weight: 44.0kDa; 44kDa
• NCBI Official Full Name: granulocyte-macrophage colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: Csf2
• NCBI Official Synonym Symbols: Csfgm; GMCSF; Gm-CSf; MGI-IGM
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: Csf2
• UniProt Synonym Gene Names: Csfgm; GM-CSF; CSF