Complement Component 1, Q Subcomponent A (C1qA) Active Protein | C1qA active protein

Active Complement Component 1, Q Subcomponent A (C1qA)

Cat. Num. AAA149262

• Applications: Activity Assay, Cell Culture
• Purity: >90%
• Synonyms: Complement Component 1, Q Subcomponent A (C1qA); N/A; Active Complement Component 1, Q Subcomponent A (C1qA); Complement Component 1,q Subcomponent,Alpha Polypeptide; C1qA active protein

• Host: E. coli
• Purity/Purification: >90%
• Form/Format: PBS, pH 7.4, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 250 ug/mL (varies by lot)

• Applicable Applications for C1qA active protein: Activity Assay, Cell Culture
• Source: Prokaryotic expression
• Residues: Ala28~Ala245
• Tags: N-terminal His-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted isoelectric point: 9.6
• Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month.; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

WB (Western Blot)

(Sample: Recombinant C1qA, Human;Antibody: Rabbit Anti-Human C1qA Ab)

SDS-PAGE

(Sample: Active recombinant C1qA, Human)

Gene Sequencing

(Figure 2. Gene Sequencing (extract))

Bioactivity

(Figure. The binding activity of C1qA with CRP.The complement component 1q (C1q) is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Complement Component 1, Q Subcomponent A (C1qA) is six A-chains of C1q. Complement component 1q (C1q is a protein complex involved in the complement system, which is part of the innate immune system. C1q together with C1r and C1s form the C1 complex. It is potentially multivalent for attachment to the complement fixation sites of immunoglobulin. The sites are on the CH2 domain of IgG and, it is thought, on the CH4 domain of IgM. IgG4 cannot bind C1q, but the other three IgG types can. Besides, C Reactive Protein (CRP) has been identified as an interactor of C1qA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human C1qA and recombinant human CRP. Briefly, C1qA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to CRP-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of C1qA and CRP was shown in Figure 1, and this effect was in a dose dependent manner.)

Sequence

Product Categories/Family for C1qA active protein

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 7705753
• NCBI GeneID: 712
• NCBI GenBank Nucleotide #: NM_015991.4
• Molecular Weight: Predicted Molecular Mass: 26.8kDa; Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: complement C1q subcomponent subunit A
• NCBI Official Synonym Full Names: complement C1q A chain
• NCBI Official Symbol: C1QA
• NCBI Protein Information: complement C1q subcomponent subunit A
• UniProt Protein Name: Complement C1q subcomponent subunit A
• UniProt Gene Name: C1QA
• UniProt Entry Name: C1QA_HUMAN