Bioactivity
(Complement Component 1, Q Subcomponent A (C1qA) is a protein that plays a crucial role in the innate immune system. It is part of the C1 complex, which is the first component of the classical complement pathway. The C1 complex is composed of one molecule each of C1q, C1r, and C1s. C1q is the recognition subcomponent of the complex and and initiating the complement cascade. C1qA plays a key role in eliminating pathogens, regulating inflammatory response and maintaining immune tolerance. It is reported that C1qA can bind to the C-terminal of CALR through its gelatin-as-region, and this interaction may play an important role in regulating complement activation and cell signaling. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse C1qA and recombinant human CALR. Briefly, C1qA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse C1qA and recombinant human CALR was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Complement Component 1, Q Subcomponent A (C1qA) Active Protein | C1qA active protein
Active Complement Component 1, Q Subcomponent A (C1qA)
Gene Names
C1qa; C1q; AI255395
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Complement Component 1, Q Subcomponent A (C1qA); N/A; Active Complement Component 1, Q Subcomponent A (C1qA); Complement Component 1, Q Subcomponent A; Complement Component 1,q Subcomponent,Alpha Polypeptide4; C1qA active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Glu23~Ala245
Applicable Applications for C1qA active protein
Activity Assay, Cell Culture
Species
Mouse
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
9.6
Research Area
Immune molecule
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Complement Component 1, Q Subcomponent A (C1qA) is a protein that plays a crucial role in the innate immune system. It is part of the C1 complex, which is the first component of the classical complement pathway. The C1 complex is composed of one molecule each of C1q, C1r, and C1s. C1q is the recognition subcomponent of the complex and and initiating the complement cascade. C1qA plays a key role in eliminating pathogens, regulating inflammatory response and maintaining immune tolerance. It is reported that C1qA can bind to the C-terminal of CALR through its gelatin-as-region, and this interaction may play an important role in regulating complement activation and cell signaling. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse C1qA and recombinant human CALR. Briefly, C1qA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse C1qA and recombinant human CALR was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Bioactivity
(Complement Component 1, Q Subcomponent A (C1qA) is a protein that plays a crucial role in the innate immune system. It is part of the C1 complex, which is the first component of the classical complement pathway. The C1 complex is composed of one molecule each of C1q, C1r, and C1s. C1q is the recognition subcomponent of the complex and and initiating the complement cascade. C1qA plays a key role in eliminating pathogens, regulating inflammatory response and maintaining immune tolerance. It is reported that C1qA can bind to the C-terminal of CALR through its gelatin-as-region, and this interaction may play an important role in regulating complement activation and cell signaling. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse C1qA and recombinant human CALR. Briefly, C1qA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse C1qA and recombinant human CALR was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for C1qA active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,974 Da
NCBI Official Full Name
complement C1q subcomponent subunit A
NCBI Official Synonym Full Names
complement component 1, q subcomponent, alpha polypeptide
NCBI Official Symbol
C1qa
NCBI Official Synonym Symbols
C1q; AI255395
NCBI Protein Information
complement C1q subcomponent subunit A
UniProt Protein Name
Complement C1q subcomponent subunit A
UniProt Gene Name
C1qa
UniProt Entry Name
C1QA_MOUSE
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Product Notes
The C1qA c1qa (Catalog #AAA161911) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Complement Component 1, Q Subcomponent A (C1qA) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the C1qA c1qa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Glu23~Ala2 45. It is sometimes possible for the material contained within the vial of "Complement Component 1, Q Subcomponent A (C1qA), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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