Bioactivity
(Complement Component 8b (C8b), one of the three subunits of the complement component 8 (C8) protein, has only been identified in vertebrates. C8 is one of the five components (C5b, C6, C7, C8 and C9) that interact to form the MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Besides, C9 can interact with C8b, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C8b and recombinant human C9. Briefly, C8b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C8b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C8b and recombinant human C9 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.)
Complement Component 8b (C8b) Active Protein | C8b active protein
Active Complement Component 8b (C8b)
Gene Names
C8B; C82
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Complement Component 8b (C8b); N/A; Active Complement Component 8b (C8b); Complement Component 8b; Complement component 8 subunit beta4; C8b active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Cys162~His504
Applicable Applications for C8b active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
7.3
Research Area
Signal transduction, Immune molecule, Rheumatology
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Complement Component 8b (C8b), one of the three subunits of the complement component 8 (C8) protein, has only been identified in vertebrates. C8 is one of the five components (C5b, C6, C7, C8 and C9) that interact to form the MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Besides, C9 can interact with C8b, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C8b and recombinant human C9. Briefly, C8b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C8b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C8b and recombinant human C9 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.)
Bioactivity
(Complement Component 8b (C8b), one of the three subunits of the complement component 8 (C8) protein, has only been identified in vertebrates. C8 is one of the five components (C5b, C6, C7, C8 and C9) that interact to form the MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Besides, C9 can interact with C8b, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C8b and recombinant human C9. Briefly, C8b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to C9-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C8b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C8b and recombinant human C9 was shown in Figure 1, the EC50 for this effect is 0.59 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for C8b active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,047 Da
NCBI Official Full Name
complement component C8 beta chain isoform 3
NCBI Official Synonym Full Names
complement component 8, beta polypeptide
NCBI Official Symbol
C8B
NCBI Official Synonym Symbols
C82
NCBI Protein Information
complement component C8 beta chain; complement component 8 subunit beta
UniProt Protein Name
Complement component C8 beta chain
UniProt Gene Name
C8B
UniProt Entry Name
CO8B_HUMAN
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Product Notes
The C8b c8b (Catalog #AAA161841) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Complement Component 8b (C8b) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the C8b c8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Cys162~His 504. It is sometimes possible for the material contained within the vial of "Complement Component 8b (C8b), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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