Bioactivity
(Ephrin A2 (EFNA2) is a cell surface-associated protein belonging to the Ephrin family, which are involved in intercellular communication and cell localization. EFNA2 is mainly expressed in tissues including the brain, heart, lung, kidney and pancreas. And this protein is involved in regulating a variety of biological processes, including cell migration, cell differentiation, and tissue boundary formation, by interacting with Eph receptors. It aslo plays a significant role in the development and maintenance of the cardiovascular system, as well as in the regulation of synaptic plasticity and neuronal circuitry in the brain. A Disintegrin And Metalloprotease 10 (ADAM10), a disintegrin and metalloproteinase, can cleave EFNA2, potentially altering the activity of EFNA2 or its interaction with Eph receptors, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA2 and recombinant rat ADAM10. Briefly, biotin-linked EFNA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ADAM10-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of EFNA2 and ADAM10 was shown in Figure 1, the EC50 for this effect is 1.38 ug/mL.)
Ephrin A2 (EFNA2) Active Protein | EFNA2 active protein
Active Ephrin A2 (EFNA2)
Gene Names
EFNA2; ELF-1; EPLG6; LERK6; HEK7-L; LERK-6
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Ephrin A2 (EFNA2); N/A; Active Ephrin A2 (EFNA2); Ephrin A2; ELF-1; EPLG6; HEK7-L; LERK6; EPH-related receptor tyrosine kinase ligand 6; HEK7 ligand4; EFNA2 active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Asn45~Ser213
Applicable Applications for EFNA2 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.3
Research Area
Neuro science
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Ephrin A2 (EFNA2) is a cell surface-associated protein belonging to the Ephrin family, which are involved in intercellular communication and cell localization. EFNA2 is mainly expressed in tissues including the brain, heart, lung, kidney and pancreas. And this protein is involved in regulating a variety of biological processes, including cell migration, cell differentiation, and tissue boundary formation, by interacting with Eph receptors. It aslo plays a significant role in the development and maintenance of the cardiovascular system, as well as in the regulation of synaptic plasticity and neuronal circuitry in the brain. A Disintegrin And Metalloprotease 10 (ADAM10), a disintegrin and metalloproteinase, can cleave EFNA2, potentially altering the activity of EFNA2 or its interaction with Eph receptors, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA2 and recombinant rat ADAM10. Briefly, biotin-linked EFNA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ADAM10-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of EFNA2 and ADAM10 was shown in Figure 1, the EC50 for this effect is 1.38 ug/mL.)
Bioactivity
(Ephrin A2 (EFNA2) is a cell surface-associated protein belonging to the Ephrin family, which are involved in intercellular communication and cell localization. EFNA2 is mainly expressed in tissues including the brain, heart, lung, kidney and pancreas. And this protein is involved in regulating a variety of biological processes, including cell migration, cell differentiation, and tissue boundary formation, by interacting with Eph receptors. It aslo plays a significant role in the development and maintenance of the cardiovascular system, as well as in the regulation of synaptic plasticity and neuronal circuitry in the brain. A Disintegrin And Metalloprotease 10 (ADAM10), a disintegrin and metalloproteinase, can cleave EFNA2, potentially altering the activity of EFNA2 or its interaction with Eph receptors, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA2 and recombinant rat ADAM10. Briefly, biotin-linked EFNA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ADAM10-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of EFNA2 and ADAM10 was shown in Figure 1, the EC50 for this effect is 1.38 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for EFNA2 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,878 Da
NCBI Official Full Name
ephrin-A2
NCBI Official Synonym Full Names
ephrin-A2
NCBI Official Symbol
EFNA2
NCBI Official Synonym Symbols
ELF-1; EPLG6; LERK6; HEK7-L; LERK-6
NCBI Protein Information
ephrin-A2; HEK7 ligand; eph-related receptor tyrosine kinase ligand 6
UniProt Protein Name
Ephrin-A2
UniProt Gene Name
EFNA2
UniProt Synonym Gene Names
EPLG6; LERK6; LERK-6; HEK7-L
UniProt Entry Name
EFNA2_HUMAN
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Product Notes
The EFNA2 efna2 (Catalog #AAA161915) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ephrin A2 (EFNA2) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the EFNA2 efna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Asn45~Ser2 13. It is sometimes possible for the material contained within the vial of "Ephrin A2 (EFNA2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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