FGF1 active protein

FGF1 Protein, Mouse, Rat, Recombinant

Cat. Num. AAA258139

• Gene Names: Fgf1; Fam; Fgfa; Dffrx; Fgf-1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: FGF1; N/A; FGF1 Protein, Mouse, Rat, Recombinant; Mouse/Rat aFGF/FGF1 Protein; fibroblast growth factor 1 (acidic); FGF1 active protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Na2SO4, 0.5mM DTT, 1mM EDTA, 20mM Tris, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe16-Asp155

• Species: Mouse, Rat
• Activity: Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.04-0.2ng/mL.
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of mouse/rat FGF1 (NP_034327.1)(Phe16-Asp155) was expressed. Mouse and Rat FGF1 sequences are identical.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.04-0.2 ng/mL.)

SDS-PAGE

Related Product Information for FGF1 active protein

Background: aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1-4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

References

Product Categories/Family for FGF1 active protein

Actin Dynamics Signaling Pathway

References

Jaye M., et al.,(1986), Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization. Science 233:541-545.Mergia A., et al., (1989), Structural analysis of the gene for human acidic fibroblast growth factor.Biochem. Biophys. Res. Commun. 164:1121-1129.Wang W.P., et al.,(1989), Cloning of the gene coding for human class 1 heparin-binding growth factor and its expression in fetal tissues.Mol. Cell. Biol. 9:2387-2395.

NCBI and Uniprot Product Information

• NCBI GI #: 6753850
• NCBI GeneID: 14164
• NCBI Accession #: NP_034327.1
• NCBI GenBank Nucleotide #: NM_010197.3
• UniProt Accession #: P61148; P10935
• Molecular Weight: 17,418 Da
• NCBI Official Full Name: fibroblast growth factor 1
• NCBI Official Synonym Full Names: fibroblast growth factor 1
• NCBI Official Symbol: Fgf1
• NCBI Official Synonym Symbols: Fam; Fgfa; Dffrx; Fgf-1
• NCBI Protein Information: fibroblast growth factor 1; HBGF-1; aFGF; acidic fibroblast growth factor; fibroblast growth factor 1 (acidic); heparin-binding growth factor 1
• UniProt Protein Name: Fibroblast growth factor 1
• UniProt Gene Name: Fgf1
• UniProt Synonym Gene Names: Fgf-1; Fgfa; FGF-1; aFGF; HBGF-1
• UniProt Entry Name: FGF1_MOUSE