Host
E Coli
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from 0.2 um filtered solution in PBS (pH 7.0) and 1mM DTT
Sequence Length
208
Bioactivity
The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05-0.5 ng/ml.
Endotoxin Level
<1.0 EU/ug of recombinant protein as determined by the LAL method.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Preparation and Storage
The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Related Product Information for FGF10 active protein
FGF-10 is involved in the initial budding as well as the continuous outgrowth of vertebrate limbs. FGF-10 mRNA is expressed preferentially in neurons but not in glial cells and may have a distinct role in the brain. Human FGF-10 is mitogenic for fetal rat keratinizing epidermal cells but not for NIH 3T3 cells. Recombinant FGF-10 induces the proliferation of human urothelial cells in vitro and induces the proliferation of transitional epithelium. FGF-10 is secreted by cultured mouse pre-adipocytes, prevention of FGF-10 signaling inhibits subsequent differentiation. The ability of embryonic fibroblasts derived from FGF-10 knock-out mice to differentiate into adipocytes is also impaired.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
19.0 kDa
NCBI Official Full Name
fibroblast growth factor 10
NCBI Official Synonym Full Names
fibroblast growth factor 10
NCBI Official Symbol
FGF10
NCBI Protein Information
fibroblast growth factor 10
UniProt Protein Name
Fibroblast growth factor 10
UniProt Gene Name
FGF10
UniProt Synonym Gene Names
FGF-10
UniProt Entry Name
FGF10_HUMAN
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Product Notes
The FGF10 fgf10 (Catalog #AAA214219) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FGF10, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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