Bioactivity
(Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen Alpha Chain (FGA) has been identified as an interactor of FGB. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGB and recombinant mouse FGA. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGA-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FGB and recombinant mouse FGA was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Fibrinogen Beta Chain (FGB) Active Protein | FGB active protein
Active Fibrinogen Beta Chain (FGB)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Fibrinogen Beta Chain (FGB); N/A; Active Fibrinogen Beta Chain (FGB); Fibrinogen Beta Chain; FG-B; Fibrinopeptide B; Fibrinogen Beta4; FGB active protein
Host
E Coli
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Gly45~Gln491
Applicable Applications for FGB active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
7.6
Research Area
Signal transduction, Infection immunity, Hematology
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen Alpha Chain (FGA) has been identified as an interactor of FGB. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGB and recombinant mouse FGA. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGA-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FGB and recombinant mouse FGA was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
Bioactivity
(Fibrinogen Beta Chain (FGB) is the beta subunit of the coagulation factor fibrinogen, which is a component of the blood clot. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. Fibrinogen Alpha Chain (FGA) has been identified as an interactor of FGB. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGB and recombinant mouse FGA. Briefly, FGB was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGA-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FGB and recombinant mouse FGA was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for FGB active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,928 Da
NCBI Official Full Name
fibrinogen beta chain isoform 2 preproprotein
NCBI Official Synonym Full Names
fibrinogen beta chain
NCBI Official Symbol
FGB
NCBI Protein Information
fibrinogen beta chain; fibrinogen, B beta polypeptide
UniProt Protein Name
Fibrinogen beta chain
UniProt Gene Name
FGB
UniProt Entry Name
FIBB_HUMAN
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Product Notes
The FGB fgb (Catalog #AAA161833) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fibrinogen Beta Chain (FGB) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the FGB fgb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Gly45~Gln4 91. It is sometimes possible for the material contained within the vial of "Fibrinogen Beta Chain (FGB), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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