Bioactivity
(Fibroblast Growth Factor 12 (FGF12) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. Fibroblast Growth Factor 12 (FGF12) may represent an important modulator of neuronal network activity and has been associated with developmental and epileptic encephalopathy (DEE). Besides, it is reported that both domains of (Ca2)4-CaM directly bind two sites in the N-terminal domain (NTD) of A-type FGF splice variants (FGF11A, FGF12A, FGF13A, and FGF14A) with high affinity. Calmodulin 1 (CALM1) has been identified as an interactor of FGF12, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF12 and recombinant human CALM1. Briefly, FGF12 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALM1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF12 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF12 and recombinant human CALM1 was shown in Figure 1, the EC50 for this effect is 4.44 ug/mL.)
Fibroblast Growth Factor 12 (FGF12) Active Protein | FGF12 active protein
Active Fibroblast Growth Factor 12 (FGF12)
Gene Names
FGF12; FHF1; FGF12B
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Synonyms
Fibroblast Growth Factor 12 (FGF12); N/A; Active Fibroblast Growth Factor 12 (FGF12); Fibroblast Growth Factor 12; FGF12B; FHF1; Fibroblast Growth Factor Homologous Factor 1; Myocyte-Activating Factor4; FGF12 active protein
Host
E Coli
Purity/Purification
Greater than 95% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Met1~Thr181
Applicable Applications for FGF12 active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Prokaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
8.6
Research Area
Cytokine
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(Fibroblast Growth Factor 12 (FGF12) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. Fibroblast Growth Factor 12 (FGF12) may represent an important modulator of neuronal network activity and has been associated with developmental and epileptic encephalopathy (DEE). Besides, it is reported that both domains of (Ca2)4-CaM directly bind two sites in the N-terminal domain (NTD) of A-type FGF splice variants (FGF11A, FGF12A, FGF13A, and FGF14A) with high affinity. Calmodulin 1 (CALM1) has been identified as an interactor of FGF12, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF12 and recombinant human CALM1. Briefly, FGF12 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALM1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF12 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF12 and recombinant human CALM1 was shown in Figure 1, the EC50 for this effect is 4.44 ug/mL.)
Bioactivity
(Fibroblast Growth Factor 12 (FGF12) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. Fibroblast Growth Factor 12 (FGF12) may represent an important modulator of neuronal network activity and has been associated with developmental and epileptic encephalopathy (DEE). Besides, it is reported that both domains of (Ca2)4-CaM directly bind two sites in the N-terminal domain (NTD) of A-type FGF splice variants (FGF11A, FGF12A, FGF13A, and FGF14A) with high affinity. Calmodulin 1 (CALM1) has been identified as an interactor of FGF12, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF12 and recombinant human CALM1. Briefly, FGF12 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALM1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF12 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF12 and recombinant human CALM1 was shown in Figure 1, the EC50 for this effect is 4.44 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for FGF12 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
181
NCBI Official Full Name
Fibroblast growth factor 12
NCBI Official Synonym Full Names
fibroblast growth factor 12
NCBI Official Symbol
FGF12
NCBI Official Synonym Symbols
FHF1; FGF12B
NCBI Protein Information
fibroblast growth factor 12; FHF-1; FGF-12; myocyte-activating factor; fibroblast growth factor 12B; fibroblast growth factor FGF-12b; fibroblast growth factor homologous factor 1
UniProt Protein Name
Fibroblast growth factor 12
UniProt Gene Name
FGF12
UniProt Synonym Gene Names
FGF12B; FHF1; FGF-12; FHF-1
UniProt Entry Name
FGF12_HUMAN
Similar Products
Product Notes
The FGF12 fgf12 (Catalog #AAA161897) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fibroblast Growth Factor 12 (FGF12) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the FGF12 fgf12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1~Thr18 1. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 12 (FGF12), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.