Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with FBLN2.)
Fibulin 2 (FBLN2) Active Protein | FBLN2 active protein
Active Fibulin 2 (FBLN2)
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with FBLN2.)
Bioactivity
(Figure 2. Inhibition of A549 cells proliferation after stimulated with FBLN2.)
Bioactivity
(Fibulin 2 (FBLN2) is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined. Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin. It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors. To test the effect of FBLN2 on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant mouse FBLN2 diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1h at 37 degree C. Proliferation of A549 cells after incubation with FBLN2 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant mouse FBLN2 for 72h. The result was shown in Figure 2. It was obvious that FBLN2 significantly inhibit cell viability of A549 cells. The ED50 is 1.25 ug/mL.)
Bioactivity
(Fibulin 2 (FBLN2) is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined. Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin. It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors. To test the effect of FBLN2 on cell apoptosis, A549 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant mouse FBLN2 diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1h at 37 degree C. Proliferation of A549 cells after incubation with FBLN2 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant mouse FBLN2 for 72h. The result was shown in Figure 2. It was obvious that FBLN2 significantly inhibit cell viability of A549 cells. The ED50 is 1.25 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
NCBI and Uniprot Product Information
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Product Notes
The FBLN2 fbln2 (Catalog #AAA161909) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Fibulin 2 (FBLN2) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the FBLN2 fbln2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Val896~Glu 1106. It is sometimes possible for the material contained within the vial of "Fibulin 2 (FBLN2), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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