Galectin 12 (GAL12) Active Protein | GAL12 active protein

Active Galectin 12 (GAL12)

Cat. Num. AAA152176

• Applications: Activity Assay, Cell Culture
• Purity: Greater than 90% by SDS-PAGE
• Synonyms: Galectin 12 (GAL12); N/A; Active Galectin 12 (GAL12); GAL12/Galectin 12; LGALS12; GRIP1; Lectin,Galactoside-Binding Soluble 12; Galectin-related inhibitor of proliferation; GAL12 active protein

• Host: E Coli
• Purity/Purification: Greater than 90% by SDS-PAGE
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.; Freeze-dried powder
• Sequence: Met1~Tyr314

• Applicable Applications for GAL12 active protein: Activity Assay, Cell Culture
• Species: Pig
• Isoelectric Point: 9.4
• Tag: N-terminal His Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.

Bioactivity

(Galectin-12 is a member of a family of mammalian lectins known as galectins. The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities. Galectin-12 is predominantly expressed in adipose tissue and detected also in macrophages and other leukocytes. It plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Po GAL12 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL12 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL12, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL12 is 0.195 ug/mL.)

SDS-PAGE

(SDS-PAGE)

Product Categories/Family for GAL12 active protein

Metabolic pathway; Active proteins/AP

NCBI and Uniprot Product Information

• NCBI GI #: 218664495
• NCBI GeneID: 100217395
• NCBI Accession #: NP_001136316.1
• NCBI GenBank Nucleotide #: NM_001142844.1
• UniProt Accession #: B7U2G9; F1RPX8
• Molecular Weight: 35,158 Da
• NCBI Official Full Name: galectin-12
• NCBI Official Symbol: LGALS12
• NCBI Protein Information: galectin-12
• UniProt Protein Name: Galectin
• UniProt Gene Name: LGALS12