Galectin 7 (GAL7) Active Protein | GAL7 active protein

Active Galectin 7 (GAL7)

Cat. Num. AAA152173

• Applications: Activity Assay, Cell Culture
• Purity: Greater than 95% by SDS-PAGE
• Synonyms: Galectin 7 (GAL7); N/A; Active Galectin 7 (GAL7); GAL7/Galectin 7; LGALS7; PIG1; TP53I1; HKL-14; Lectin Galactoside-Binding Soluble 7; p53-induced gene 1 protein; GAL7 active protein

• Host: E Coli
• Purity/Purification: Greater than 95% by SDS-PAGE
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.; Freeze-dried powder
• Sequence: Met1~Val128

• Applicable Applications for GAL7 active protein: Activity Assay, Cell Culture
• Species: Rat
• Isoelectric Point: 6.6
• Tag: N-terminal His Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month, -80 degree C for 12 months; Stable for 12 months if correctly stored.

Bioactivity

(The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re-epithelialization of woundsIt can agglutinate red blood.In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Ra GAL7 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL7 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL7, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL7 is 25 ug/mL.)

SDS-PAGE

(SDS-PAGE)

Product Categories/Family for GAL7 active protein

Tumor immunity; Active proteins/AP

NCBI and Uniprot Product Information

• NCBI GI #: 158517925
• NCBI GeneID: 29518
• NCBI Accession #: NP_072104.2
• NCBI GenBank Nucleotide #: NM_022582.2
• UniProt Accession #: P97590; O54958
• Molecular Weight: 15,285 Da
• NCBI Official Full Name: galectin-7
• NCBI Official Synonym Full Names: galectin 7
• NCBI Official Symbol: Lgals7
• NCBI Protein Information: galectin-7
• UniProt Protein Name: Galectin-7
• UniProt Gene Name: Lgals7
• UniProt Synonym Gene Names: Gal-7